小鼠对表达HBV截短肽的耻垢分枝菌的免疫应答  

Immune Response Elicited by Recombinant Mycobacterium smegmatis Expressed HBV Fused Gene in Mice in vivo

作　　者：胡兴斌[1] 尹文[1] 韦三华[1] 雷迎峰[1] 吕欣[1] 杨敬[1] 孙梦宁[1] 徐志凯[1] 

机构地区：[1]第四军医大学微生物学教研室,陕西西安710032

出　　处：《微生物学杂志》2006年第5期26-29,共4页Journal of Microbiology

基　　金：国家自然科学基金资助项目(30671878)

摘　　要：分别以生理盐水、pcDNA3.1(-)空质粒、重组真核质粒pcDNA3.1(-)-C-preS1(简称基因免疫组)、耻垢分枝杆菌和重组耻垢分枝杆菌(简称重组耻垢免疫组)注射BALB/c小鼠,各组均免疫2次,每次间隔3周。末次注射后进行连续抗体测定,最后处死小鼠分离脾细胞,进行淋巴细胞增殖实验和细胞毒性T细胞杀伤实验。重组耻垢免疫组抗体滴度升高幅度前期低于基因免疫组(P<0.05),后者45 d达到峰值1∶5 100。前者抗体滴度第60 d达到峰值1∶6 900,45 d后重组耻垢免疫组抗体滴度高于基因免疫组(P<0.05),且维持时间较基因免疫组更长(P<0.05)。基因免疫组诱导的小鼠脾淋巴细胞增殖刺激指数低于重组耻垢免疫组(P<0.05)。后者诱导的特异性CTL杀伤率最高可达到50.2%,而前者最高为31.6%,二者有显著区别(P<0.05)。Saline, empty pcDNA3.1（-） plasmids, recombinant eukaryotic plasmids of pcDNA3.1（-）-C-preS1, Mycobacterium smegmatis, and recombinant M. smegmatis were administrated to BALB/c mice respectively with a three weeks interval. Then antibody titer, spleen cell proliferation and CTL effects were evaluated. In early stage （P 〈 0.05）, DNA immunization group witnessed more rapid increase of antibody and peaked at 1：5 100. But it lagged behind the group immunized with recombinant M. smegmatis 45 days later and had more apparent decrease trend （P〈 0.05）, and the later climbed to 1：6 900 on the day of 60. There were obvious differences in lymphocyte proliferation between DNA immunization and M. smegmatis immunization（P〈 0.05）. The later elicited a higher proliferation index, 2.21. CTL introduced specific cell lysis rate could reach up to 50.2 % in the M. smegmatis immunized group, while that of DNA immunized group was 31.6 %.

关 键 词：乙型肝炎病毒 细胞毒性T细胞 耻垢分枝杆菌 抗体 

分 类 号：R373.2[医药卫生—病原生物学]