Evaluation of the damage in fish spermatozoa cryopreservation  

作　　者：李军 刘清华 张士璀 

机构地区：[1]Institute of Oceanology, Chinese Academy of Sciences [2]Department of Marine Biology, Ocean University of China

出　　处：《Chinese Journal of Oceanology and Limnology》2006年第4期370-377,共8页中国海洋湖沼学报（英文版）

基　　金：Funded by the National High Technology R&D Program of China (863 Program, Nos. 2001AA621100, 2003AA603510 and 2004AA603310).

摘　　要：Cryodamages occur during sperm cryopreservation. Cryopreservation of fish sperm usually results in marked decrease in sperm quality, such as swelling or disruption of the plasma membrane, mi-tochondrial dysfunction, diminished sperm motility, impaired velocity, shorter motility period, denatura-tion, and release of some enzymes from spermatozoa. In this paper, damages in morphology, physiology, biochemistry and metabolism, and genetic integrity of fish semen after cryopreservation are discussed. New approaches in assessment of fish thawed sperm quality such as computer assisted sperm analysis, flow cytometic analysis combined with fluorescent probes and single cell gel electrophoresis are also briefly reviewed.Cryodamages occur during sperm cryopreservation. Cryopreservation of fish sperm usually results in marked decrease in sperm quality, such as swelling or disruption of the plasma membrane, mitochondrial dysfunction, diminished sperm motility, impaired velocity, shorter motility period, denaturation, and release of some enzymes from spermatozoa. In this paper, damages in morphology, physiology, biochemistry and metabolism, and genetic integrity of fish semen after cryopreservation are discussed. New approaches in assessment of fish thawed sperm quality such as computer assisted sperm analysis, flow cytometic analysis combined with fluorescent probes and single cell gel electrophoresis are also briefly reviewed.

关 键 词：cryodamage CRYOPRESERVATION fish sperm freezing and thawing 

分 类 号：S917.4[农业科学—水产科学]