Immunocytochemical localization of neuropeptide Y,serotonin,substance P and β-endorphin in optic ganglia and brain of Metapenaeus ensis  

作　　者：叶海辉 王桂忠 金朱兴 黄辉洋 李少菁 

机构地区：[1]Department of Oceanography, Xiamen University

出　　处：《Chinese Journal of Oceanology and Limnology》2006年第4期384-389,共6页中国海洋湖沼学报（英文版）

基　　金：Supported by the Key Foundation Research Program of Fujian Province (1998-2002).

摘　　要：By using immunocytochemistry method of Strept Avidin-Biotin-Complex, four kinds of antisera raised against rabbits were applied to observe the immunoreactive neurons and neuropils of sero-tonin (5-HT), neuropeptide Y (NPY), substance P (SP) and β-Endorphin (β-Ep) in optic ganglia and brain of Metapenaeus ensis. The results showed that, the 5-HT-immunoreactive cells were located in all the four neuropils of optic ganglia. Immunoreactivity of 5-HT was detected in anterior medial protocerebrum neuropils (AMPN), and the inner and outer lateral beside olfactory lobe (OL) of deutocerebrum. The presence of NPY-immunoreactive cells was found in all the four neuropils of the optic ganglia. NPY-immunoreactivity occurred in the anterior median cell cluster, lateral cell cluster of protocerebrum, and cell cluster beside OL and AMPN. SP-immunoreactivity was found in medulla terminalis (MT) of optic ganglia, and lateral cell cluster of protocerebrum and posterior lateral cell cluster of tritocerebrum. β-Ep-immunoreactive cells were in MT only. In conclusion, these specific distribution patterns of the four immunoreactive substances can be used as morphological clues for understanding their different neuro-physiological functions.By using immunocytochemistry method of Strept Avidin-Biotin-Complex, four kinds of antisera raised against rabbits were applied to observe the immunoreactive neurons and neuropils of serotonin （5-HT）, neuropeptide Y （NPY）, substance P （SP） and β-Endorphin （β-Ep） in optic ganglia and brain of Metapenaeus ensis. The results showed that, the 5-HT-immunoreactive cells were located in all the four neuropils of optic ganglia. Immunoreactivity of 5-HT was detected in anterior medial protocerebrum neuropils （AMPN）, and the inner and outer lateral beside olfactory lobe （OL） of deutocerebrum. The presence of NPY-immunoreactive cells was found in all the four neuropils of the optic ganglia. NPY-immunoreactivity occurred in the anterior median cell cluster, lateral cell cluster of protocerebrum, and cell cluster beside OL and AMPN. SP-immunoreactivity was found in medulla terminalis （MT） of optic ganglia, and lateral cell cluster of protocerebrum and posterior lateral cell cluster of tritocerebrum. β-Ep-immunoreactive cells were in MT only. In conclusion, these specific distribution patterns of the four immunoreactive substances can be used as morphological clues for understanding their different neuro- physiological functions.

关 键 词：Metapenaeus ensis  optic ganglia BRAIN IMMUNOCYTOCHEMISTRY 

分 类 号：S917.4[农业科学—水产科学]