一种新型海洋纤溶酶体外抗凝与溶栓作用研究  

Study on anticoagulant and thrombolytic effects of a novel marine fibrinolytic enzyme in vitro

作　　者：王佃亮[1] 刘万顺[1] 韩宝芹[1] 许瑞安[2] 张海智 刘学英 

机构地区：[1]中国海洋大学海洋生命学院,山东青岛266003 [2]华侨大学分子药物研究所,福建泉州362021 [3]潍坊大学生物工程学院,山东潍坊261061

出　　处：《中国海洋药物》2006年第4期37-42,共6页Chinese Journal of Marine Drugs

基　　金：国家863高技术发展计划项目(No.2001AA6250502002AA216051No.2005AA216060);国家"十五"科技攻关项目(No.2001BA708B04-07)

摘　　要：目的对本实验室制备的一种相对低分子质量新型海洋纤溶酶的体外抗凝和溶栓作用进行初步评价,为进一步的动物实验研究打下基础。方法以pH7.4的生理盐水作阴性对照,以蚓激酶作阳性对照,在体外研究新型海洋纤溶酶对Wistar大鼠和家兔新鲜血液的抗凝集和血栓溶解作用。结果该新型海洋动物纤溶酶具有显著的血栓溶解作用,并具有一定的抗凝血效果,从血栓中释放的血细胞形态较使用蚓激酶优。结论该新型海洋纤溶酶具有潜在的应用价值。Objective To estimate the anticoagulant and thrombolytic effects of a novel fibrinolyric enzyme with a low molecular weight for further carrying on animal test and clinic research. Methods Using pH7.4, 0.9 % NaCl as negative control and lumbrukinase as positive control, the anticoagulant and thrombolytic effects of the novel marine fibrinolytic enzyme were investigated in vitro with fresh blood from wistar rat and rabbit, respectively. Results The results showed that there were apparent anticoagulant effect as well as thrombolytic effects in the self-made marine fibrinolytic enzyme, and the status of the released blood cells from thrombus using this enzyme was also better than that of using lumbrukinase. Conclusion The novel self-made fibrinolytic enzyme is a potent thrombolytic agent.

关 键 词：海洋纤溶酶 抗凝血活性 溶栓作用 

分 类 号：R931.77[医药卫生—生药学] R973.2[医药卫生—药学]