氯霉素单克隆抗体免疫金标快速检测试纸的研制及性能测定  

Development and Trait Identification of Colloidal Gold-Labeled Monoclonal Antibody Strip for Rapid Detection of Chloramphenicol

作　　者：王自良[1] 张改平[2] 杨艳艳[2] 张海棠[1] 杨继飞[2] 刑广旭[2] 柴书军[2] 

机构地区：[1]河南科技学院动物科学学院,河南新乡453003 [2]河南省农业科学院生物技术研究所,河南郑州450002

出　　处：《华北农学报》2006年第5期127-131,共5页Acta Agriculturae Boreali-Sinica

基　　金：国家"863"项目资助(2001AA249030)

摘　　要：以分泌抗氯霉素单克隆抗体(CAP mAb)杂交瘤细胞株的建立为基础,应用胶体金免疫层析试验(GICA)成功研制出CAP残留快速检测试纸(CAP_Strip),并对其性能进行了测定。结果表明,CAP_Strip的标准曲线呈典型的S型,符合4参数logit曲线拟合,目测检测限为0.5μg/L;与氯霉素琥珀酸钠的交叉反应为160%,与其他酰胺醇类和抗菌药物无交叉反应;其敏感性与竞争ELISA试剂盒相当,符合率为100%;10 min内显示结果,具有快速、敏感、特异、简便等特点,适合CAP残留快速检测的推广应用。Based on the generation of monoclonal antibody against Chloramphenicol （CAP mAb）, the rapid test strip of CAP（CAP-Strip） was developed with colloidal gold immunochromatography assay（GICA）and its traits were tested. The result showed that the calibration curve of CAP-Strip was typical sigmoid curve which fitted the four parameter logistic, with a eyeballing detection limit of 0.5 μg/L. It generally had 160 % cross-reactivity towards CAP succinate sodium, and little or no cross-reactivity with other amphenicols and antibacterials. Its sensitivity was the same as that of competitive ELISA-Kit, with a coincidence rate of 100%. The strip could give the test results in less than 10rain and possessed good sensitivity, specificity and briefness, which was proved to be suitable for the rapid test of CAP residues in animal food.

关 键 词：氯霉素 单克隆抗体 胶体金免疫层析试验 快速检测试纸 

分 类 号：S859.84[农业科学—临床兽医学]