HPLC法测定清咽胶囊中穿心莲内酯和脱水穿心莲内酯的含量  

Determination of Andrographolide and Dehyandrogra Pholide in Qingyan Capsules by HPLC

作　　者：卢华[1] 李修善 陈英[1] 郭炎荣[1] 

机构地区：[1]广西壮族自治区人民医院,南宁530021 [2]广西万寿堂药业有限公司,南宁530219

出　　处：《海峡药学》2006年第5期65-67,共3页Strait Pharmaceutical Journal

摘　　要：目的建立清咽胶囊中穿心莲内酯和脱水穿心莲内酯的含量测定方法。方法应用HPLC法，选用ReliesilCls色谱柱（250×4．6mm，5μm），流动相为甲醇-水（65：35），流速为：1ml·min^-1，检测波长为250nm。结果本品中穿心莲内酯含量测定线性范围为0．084-0．756μg（r=0．9999），平均回收率为97．5％（RSD=2．26％，n=5）；脱水穿心莲内酯含量测定线性范围为0．036～0．324μg（r=0．9998），平均回收率为100．1％（RSD=0．86％，n=5）。结论含量测定方法准确可行，重复性好，作为药品标准可有效地控制清咽胶囊的质量。OBJECTIVE To establish a determination of Andrographolide and Dehyandro grapholide in Qingyan Capsules. METHODS Reliesil C18 column （5μm, 250 × 4.6mm） was used. The mobile phase was consisted of methanol-water （65 ： 35） and the measuring wavelength was 250nm. RESULTS Linear range of Andrographolide was 0. 084 to 0. 756ttg（r=0. 9999）,The average recovery was 97.5% （RSD= 2.26% n= 5）; and the dehydroandrographolide was 0. 036 to 0. 324 μg（r=0. 9998）,The average recovery was 100.1% （RSD =0. 86% n= 5）. CONCLUSION The method of quantification were accurate, realizible and reproductable. It can be used effectively for the quality control of Qingyan Capsules.

关 键 词：清咽胶囊 穿心莲 穿心莲内酯 脱水穿心莲内酯 高效液相色谱 

分 类 号：R927.2[医药卫生—药学]