罗氏调经种子丸治疗肾虚肝郁型黄体不健的临床研究  

Therapeutic Effect ofLuoshi Tiaojing ZhongziPill on Luteal Phase Defect with Kidney Deficiency and Liver Stagnation

作　　者：张玉珍[1] 史云[1] 罗颂平[1] 许丽绵[1] 张晶[1] 曾锦蕙[1] 黄晓燕[1] 杜莹[1] 

机构地区：[1]广州中医药大学第一附属医院妇科,广州510405

出　　处：《广州中医药大学学报》2006年第6期457-460,464,共5页Journal of Guangzhou University of Traditional Chinese Medicine

摘　　要：【目的】研究罗氏调经种子丸对肾虚肝郁型黄体不健的治疗作用及机理。【方法】观察肾虚肝郁型黄体不健所致月经失调患者79例,给予罗氏调经种子丸(主要成分为菟丝子、熟地、当归、白芍、淮山药、柴胡等)治疗3个月,观察疗效;采用酶标法测定治疗前后血清卵泡刺激素(FSH)、黄体生成素(LH)、雌二醇(E2)、睾酮(T)、催乳素(PRL)、孕酮(P)水平,并比较治疗前后基础体温(BBT)的变化。采用灭吐灵注射法复制高泌乳素血症的黄体功能不全大鼠模型进行动物实验,观察罗氏调经种子丸对模型大鼠体质量、子宫和卵巢系数、内分泌水平、成熟卵泡数、新生黄体数、闭锁卵泡数及子宫内膜的影响。【结果】79例月经失调患者中,有效65例,无效14例,总有效率达82.28%;治疗前后血清FSH、LH、E2、PRL、P水平及LH与FSH比值均有显著性差异(P<0.01);治疗后BBT在卵泡期和黄体期均有显著性改善(P<0.01)。动物实验结果显示:罗氏调经种子丸能够增加大鼠体质量和子宫、卵巢系数,升高E2、P水平,降低过高的PRL水平,增加成熟卵泡数、新生黄体数(P<0.05或P<0.01)。【结论】罗氏调经种子丸治疗肾虚肝郁型黄体不健有良好的疗效,其机制可能与调整内分泌,促进卵泡发育和改善黄体功能的作用有关。[ Objective] To observe the effect of Luoshi Tiaojing Zhongzi Pill （LTZP） on luteal phase defect with kidney deficiency and liver stagnation and to explore its therapeutic mechanism. [ Methods] Ninety-six patients with menstrual disorder, which was caused by luteal phase defect with kidney deficiency and liver stagnation, were enrolled into the study. Seventy-nine patients in group A received LZTP （mainly composed of Semen Cuscutae, Radix Rehmanniae Preparata, Radix Angelicae Sinensis, Radix Paeoniae Alba, Rhizoma Dioscoreae, Radix Bupleuri, etc. ） for 3 months, and 17 patients in group B received Qiling Pill. After treatment, the therapeutic effect in the two groups was evaluated. The changes of serum levels of follicle stimulating hormone （FSH）, luteinizing hormone （LH）, estradiol （E2）, prolactin （PRL）, testosterone （T） and progesterone （P） were observed by enzyme-linked immunosorbent assay （ELISA） before and after treatment in group A. Meanwhile, the change of basic body temperature （BBT） was also observed. Imperan was used to induce the rat models of luteal phase defect with high blood prolactin. The effects of LTZP on rat body weight, coefficient of uterus and ovary, endocrine hormone levels, mature follicle number, neogenetic corpus luteum number, atretic follicle number as well as the change of endometrium were observed to explore the therapeutic mechanism of LTZP. [ Results] After treatment with LTZP, 65 patients were effective, 14 ineffective and the total effective rate was 82.28% in group A. The differences of FSH, LH, E2, PRL, P and LH/FSH were significant （P 〈 0.01） before and after treatment; BBT was improved in follicular phase and luteal phase （P 〈 0.01） after treatment. The results of the animal experiment showed that LTZP increased rat body weight, coefficient of uterus and ovary, serum E2 and P levels as well as mature follicle number and neogenetic corpus luteum number, and decreased serum PRL level （ P 〈 0.05 or P 〈 0.01 ）. [ Conclus

关 键 词：黄体不健/中药疗法 罗氏调经种子丸/治疗应用 罗氏调经种子丸/药理学 罗元恺 

分 类 号：R711.51[医药卫生—妇产科学]