雷公藤甲素对外周血单个核细胞分泌促炎、抑炎细胞因子的影响  

Influence of Triptolide A on Production of Inflammation-promoting and Anti-inflammatory Cytokines in Peripheral Blood Mononuclear Cells

作　　者：盛冬云[1] 涂胜豪[1] 陈红波[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院中西医结合科,湖北武汉430030

出　　处：《广州中医药大学学报》2006年第6期484-486,共3页Journal of Guangzhou University of Traditional Chinese Medicine

摘　　要：【目的】探讨雷公藤甲素(triptolide A,TA)对健康人外周血单个核细胞(peripheral blood mononuclear cells,PBMC)分泌促炎、抑炎细胞因子的影响。【方法】分离培养健康人PBMC,采用脂多糖(LPS,终浓度为1μg/mL)或LPS+植物血凝素(PHA,终浓度为25μg/mL)刺激培养细胞,并给予TA(终浓度为5.4 ng/mL)处理,收集培养上清液,采用酶联免疫吸附(ELISA)法检测上清液中肿瘤坏死因子α(tumour necrosis factor-,αTNF-α)、白介素4(interleukin-4,IL-4)、白介素10(interleukin-10,IL-10)的含量。【结果】TA能抑制LPS或LPS+PHA刺激的PBMC分泌TNF-α和IL-10,且对正常人PBMC分泌IL-10的抑制作用强于对TNF-α的抑制作用,但对IL-4的分泌无显著性影响。【结论】雷公藤甲素可不同程度地抑制促炎性细胞因子TNF-α及抑炎性细胞因子IL-10,使促炎、抑炎两种作用趋于平衡。[Objective] To investigate the influence of triptolide A （TA） on the production of inflammation-promoting and anti-inflammatory cytokines in peripheral blood mononuclear cells （PBMC） from the healthy volunteers. [ Methods] PBMC isolated from the healthy volunteers were cultured and stimulated by lipopolysaccharide （LPS, the final concentration being 1μg/mL） or LPS ＋ phytohemagglutinin （PHA, the final concentration being 25 μg/mL）, and were also treated with or without TA （the final concentration being 5.4 ng/mL） simultaneously. The contents of tumor necrosis factor α （TNF-α）, interleukin 4 （IL-4） and interleukin 10 （IL-10） in the supernatant of the culture were measured by enzyme-linked immunosorbent assay （ELISA）. [ Results] TA inhibited the production of TNF-α and IL-10 in PBMC culture stimulated by LPS or LPS ＋ PHA, and the inhibitory effect of TA on IL-10 was stronger than on TNF- α, but had no effect on the production of IL-4. [Conclusion] TA has an inhibitory effect on the inflammation-promoting cytokine of TNF-α and anti-inflammatory cytokine of IL-10, which keeps the balance of inflammation-promoting and anti- inflammatory actions.

关 键 词：雷公藤甲素/药理学 细胞因子类 细胞培养 

分 类 号：R285.5[医药卫生—中药学]