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作 者:厉晓腊[1] 金轶伟[1] 柴一秋[1] 陈官菊[1] 刘又高[1]
机构地区:[1]浙江省亚热带作物研究所,浙江温州325005
出 处:《茶叶科学》2006年第4期265-269,共5页Journal of Tea Science
基 金:温州市科技局资助项目(N2005A015)
摘 要:研究了茶毛虫核型多角体病毒(Euproctis pseudoconspersa nuclear polyhedrosis virus,简称EpNPV)的室内外增殖、分离粗提,以及对茶毛虫(Euproctis pseudoconspersa Strand)室内毒力测定、田间防治效果和病毒电镜检测。采用室内离体茶树嫩枝水培,10^6 PIB/ml EpNPV喷雾接种2~3龄茶毛虫,或大田直接喷雾处理2-3龄茶毛虫,在罹病幼虫液化前及时收集虫尸,完成病毒增殖。10^6 PIB/ml、10^7 PIB/ml、10^8 PIB/ml EpNPV室内处理第12天致病率分别为43.60%、76.13%和64.68%;第14天致病率分别为59.17%、95.71%和95.70%。大田试验10^7 PIB/ml喷雾处理第15天防治效果达78.11%。经透射电镜观察田间防治大量感病茶毛虫虫尸,证实为EpNPV致病死亡。The reproduction, extraction, pathogenicity to Tea Tussork Moth and electron micrograph testing of Euproctis pseudoconspersa Nuclear Polyhedrosis Virus(EpNPV) was investigated. The EpNPV was propagated through Inoculating 2-3 instar larvae of Euproctis pseudoconspersa, by raged with EpNPV at the concentration 10^6 PIB/ml, or inoculating 2-3 instar larvae in field condition. Inoculation the larvae with 10^6 PIB/ml, 10^7 PIB/ml, and 10^8 PIB/ml of EpNPV, the corrected mortalities were 43.60%, 76.13% and 64.68% on day 12 respectively, and reached 59.17%, 95.71%, and 95.70% on day 14 respectively. The mortality was 78.11% on day 15 treated with EpNPV(10^7 PIB/ml) under field condition. Virus of the EpNPV from the field dead larvae could be watched through the electron microscope.
关 键 词:茶毛虫 核型多角体病毒(EpNPV) 致病性 增殖 生物防治
分 类 号:S435.711[农业科学—农业昆虫与害虫防治]
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