昆藻调脂胶囊对实验性高脂血症性脂肪肝大鼠肝细胞增殖的影响  

Effects of Kunzao Tiaozhi Capsule on Cell Proliferation in Experimental Rats with Fatty Liver and Hyperlipidemia

作　　者：孔祥廉[1] 梅全喜[1] 钟希文[1] 高玉桥[1] 何玉萍[2] 梁毅[2] 林慧[1] 吴惠妃[1] 

机构地区：[1]广州中医药大学附属中山中医院,中山市528400 [2]广州中医药大学附属第一医院,广州市510180

出　　处：《中国药房》2006年第21期1609-1610,共2页China Pharmacy

基　　金：广东省中医药管理局科技基金资助项目(1040187)

摘　　要：目的:探讨昆藻调脂胶囊治疗高脂血症性脂肪肝的作用机制。方法:取59只大鼠随机分为正常对照组(n=10)、模型组(n=11)、阳性对照组(n=10)及昆藻调脂胶囊高、中、低剂量组(n=9、9、10),除正常对照组外,其余各组采用高脂饲料及30%乙醇方法复制脂肪肝模型,同时给予相应溶剂或药物,10wk后运用流式细胞术观察各组大鼠肝细胞DNA的S期细胞比例(SPF)及增殖指数(PI)。结果:与正常对照组比较,造模后大鼠PI、SPF值明显升高(P<0.05);昆藻调脂胶囊能降低造模后大鼠PI、SPF值(P<0.05),尤其对♀大鼠的作用更为显著(P<0.01)。结论:昆藻调脂胶囊通过抑制高血脂大鼠特别是♀大鼠肝细胞的增殖而发挥抗肝纤维化作用。OBJECTIVE： To study the action mechanism of kunzao tiaozhi capsule in the treatment of fatty liver with hyperlipidemia .METHODS： A total of 59 rats were randomly divided into normal control group（n = 10）, model group（n = 11）, positive control group（n = 10） and kunzao tiaozhi capsule group（at high, medium and low doses, n = 9,9, 10）.Rat model with fatty livers was established in all the groups except the normal control group by feeding high - fat forage and 30% alcohol, and the corresponding solvents or medicine were given during the modeling, 10 weeks later, the S - phase cell percentages（SPF） of the liver cells in rats and proliferation index（PI ）were determined by flow cytometry methods. RESULTS：As compared with the normal control group, PI and SPF values in the modeled rats increased significantly（ P 〈 0.05）, while which were decreased in kunzao tiaozhi capsule group after modeling（P 〈 0.05）, especially in the female rats（P 〈 0.01） .CONCLUSION： The antihepatic fibrosis effect of kunzao tiaozhi capsule can be achieved by inhibiting the cell proliferation in rats with hyperlipidemla （especially the female rats）.

关 键 词：昆藻调脂胶囊 高脂血症 脂肪肝 大鼠 细胞增殖 流式细胞术 

分 类 号：R285.5[医药卫生—中药学]