异氟醚对新生大鼠认知功能的影响  

Effects of isoflurane anesthesia on cognitive function in neonatal rats

作　　者：蒯建科[1] 姚立农[1] 于代华[1] 张凤林[1] 彭德民[1] 杨永慧[1] 柴伟[1] 

机构地区：[1]第四军医大学唐都医院麻醉科,西安市710038

出　　处：《中华麻醉学杂志》2006年第8期717-719,共3页Chinese Journal of Anesthesiology

基　　金：陕西省社会发展科技公关项目(2003G4-K10)

摘　　要：目的观察异氟醚对新生大鼠认知功能的影响。方法新生1周Wistar大鼠40只,雌雄不拘,随机分为5组(n=8)：对照组(A组)吸人空气,B、C组分别吸入1．8%和1．2%异氟醚6 h,D、E组分别吸人1．8%、1．2%异氟醚3次,1次/日,2h/次。吸入异氟醚后3周进行认知功能测试,包括旷场实验和Morris水迷宫测试,认知功能测试完毕后立即处死大鼠,取海马组织,进行免疫组织化学染色,测定N-甲基-D-天冬氨酸受体-2(NMDAR_2)及谷氨酸转运体(GLAST)的表达。结果吸入异氟醚可导致大鼠认知功能降低,海马NMDAR_2和GLAST表达增加,吸入高浓度异氟醚后海马NMDAR_2和GLAST表达增加更明显,单次吸入和多次吸入异氟醚后海马NMDAR_2和GLAST表达差异无统计学意义(P＞0．05)。结论异氟醚可降低新生大鼠认知功能,与上调海马NMDAR_2及GLAST表达有关。Objective To investigate the long-term changes in cognitive function and glutamatergic N-methyl-D-aspartate receptor-2 （NMDAR2 ） and GLAST expression in hippocampus after isoflurane anesthesia in neonatal rats. Methods Forty 1 week old Wistar rats of either sex were randomly divided into 5 groups （ n = 8, each） ： group A control, group B and C inhaled 1.8% and 1.2% isoflurane for 6 h respectively; group D and E inhaled 1.8 % and 1.2 % isoflurane 2 h per day for 3 days respectively. Cognitive function was assessed using open fieled and Morris water maze test at 21 d after last anesthesia. The animals were killed immediately after the tests and hippocampus was isolated for determination of NMDAR2 and GLAST expression using immuno-histochemical technique. Results Isoflurane anesthesia induced cognitive decline and increased NMDAR2 and GLAST expression in hippocampus dose-dependently. But there was no significant difference in NMDAR2 and GLAST expression between group B and D and group C and E. Conclusion Isoflurane anesthesia can induce long-lasting cognitive decline and increase the expression of glutamatergic NMDAR2 and GLAST in hippocampus in neonatal rats.

关 键 词：异氟醚 认知 受体 N-甲基-D-天冬氨酸 谷氨酸 海马 

分 类 号：R96[医药卫生—药理学]