烟草凝集物对家兔受精卵Cu，Zn－SOD酶活性的影响  

The Effect of Cigarette Smoking Condensate (CSC) on Activity of Cu,Zn SOD in Rabbit Fertilized Egg

作　　者：苏心[1,2] 王彦泓[1,2] 张静 郭凯英[1,2] 余海晖 彭敦仁 

机构地区：[1]天津市环湖医院病理科 [2]天津市计划生育研究所

出　　处：《环境与健康杂志》1996年第2期58-60,共3页Journal of Environment and Health

基　　金：国家计划生育委员会基金

摘　　要：以Ｃｕ，Ｚｎ－ＳＯＤ为标志酶，研究了烟草凝集物（ＣＳＣ）对家兔受精卵酶活性的影响。结果：２小时培养的各组Ｃｕ，Ｚｎ－ＳＯＤ酶活性分别为５３８．２Ｕ（对照组），４７３．５Ｕ（组Ⅰ），３４８．２Ｕ（组Ⅱ），１１６．４Ｕ（组Ⅲ）；４小时培养的各组Ｃｕ，Ｚｎ－ＳＯＤ酶活性分别为４３６．４Ｕ（对照组），３３２．５Ｕ（组Ⅰ），２３２．１Ｕ（组Ⅱ），５５．８Ｕ（组Ⅲ），计算各组与ＣＳＣ剂量间的相关性，相关系数分别为－０．９９６，－０．９３８，即随着ＣＳＣ量的加大，Ｃｕ，Ｚｎ－ＳＯＤ酶活性下降，结果表明ＣＳＣ对超氧化物歧化酶系统有较强烈的抑制作用。The effect of cigarette smoking condensate (csc) on rabbit fertilized egg was studied.Blastocyst was cultivated with different amount of csc,and with the Cu,Zn SOD (Supero xide dismutase) as the mark of enzyme. Results were shown as following:After 2 hrs culture,Cu,Zn SOD activities were 538.2U,473.5U,348.2U and 116.4U in the control group,group Ⅰ,Ⅱ,and Ⅲ,respectively.After 4 hrs culture,those were 436.4U,332.5U,232.1U and 55.8U, respectively.The relationship between the enzyme activity and csc dose for each group was calculated statistically,the regression coefficients were -0.996 in group Ⅰ and -0.938 in group Ⅱ respectively,i.e.,Cu,Zn SOD activity of the blastocyst decreased with the increase of csc added.Based on these results it may be concluded that SOD system in the blastocyst is inhibited strongly by csc.

关 键 词：烟草凝集物 超氧化物歧化酶 家禽 兔 受精卵 

分 类 号：X503.22[环境科学与工程—环境工程]