金黄色葡萄球菌对左心室细胞黏附分子和炎症因子基因表达的影响  

Effect of Staphylococcus aureus on expressions of cell adhesion molecules and inflammatory mediator in left ventricular myocardium

作　　者：赵波[1] 孙威[1] 付向宁[1] 陈启福[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院胸心外科,湖北武汉430030

出　　处：《心脏杂志》2006年第5期492-495,共4页Chinese Heart Journal

摘　　要：目的初步探讨金黄色葡萄球菌对左心室细胞黏附分子和炎症因子基因表达的影响和感染性心内膜炎的发病机制。方法7只健康家兔做对照,24只家兔分别经静脉注射金黄色葡萄球菌(金葡菌)和二尖瓣手术。术后24h处死,取出左心室组织,肉眼、电镜观察。RT-PCR技术检测细胞黏附分子(E-Selectin,PECAM-1,VCAM-1)和炎症因子-巨噬细胞集落刺激因子-1(MCSF-1)mRNA表达水平。结果单独金葡菌注射没有引起左心室靶基因的显著变化;二尖瓣手术后左心室组织E-Selectin,PECAM-1和MCSF-1 mRNA表达水平显著升高,而VCAM-1 mRNA没有显著的变化;二尖瓣手术并发金葡菌注射后左心室组织E-Selectin和MCSF-1 mRNA表达显著升高,而PECAM-1和VCAM-1 mRNA表达降低。结论金黄色葡萄球菌在心内膜损伤和病理血流状态下,导致左心室部分特定的细胞黏附分子和炎症因子表达变化,引起感染性心内膜炎发生。AIM To investigate how Staphylococcus aureus influences gene expressions of cell adhesion molecules （E-Selectin, PECAM-1, VCAM-1 ） and MCSF-1 in left ventricular myocardium and explore the cellular immunology mechanism of infective endocarditis. METHODS Seven rabbits received 5 × 10^6 colony forming units Staphylococcus aureus i. v. , 9 rabbits underwent mitral surgery and 8 rabbits underwent both interventions in combination. The hearts were explanted 24 h after the intervention and observed by naked eye and electron microscope, mRNA abundance was determined by semiquantitative duplex RT-PCR. Results were compared with those in control group consisting of 7 untreated rabbits. RESULTS Whereas the bacteraemia did not lead to upregulated mRNA levels of any studied molecules, mitral valve surgery caused significantly higher relative expression levels of E-Selectin, PECAM-1 and MCSF-1, but not of VCAM-1. The combination of bacteremia and mitral valve surgery caused an uneven expression pattern with a significant upregulation of E-Selectin and MCSF-1 and a downregulation of PECAM-1 and VCAM-1. CONCLUSION Staphylococcus aureus leads to changes of regulations of specific cell adhesion molecules and MCSF-1 in left ventricular myocardium after surgical procedure, thus resulting in the development of infective endocarditis.

关 键 词：心内膜炎 金黄色葡萄球菌 黏附分子 炎症因子 

分 类 号：Q256[生物学—细胞生物学] R542.41[医药卫生—心血管疾病]