灯盏花素对大鼠心肌缺血再灌注损伤诱导细胞凋亡的影响  

Effect of Breviscapine on myocyte apoptosis in myocardial ischemia-reperfusion rat model

作　　者：吴岩松[1] 李树青[1] 赵振梅[1] 

机构地区：[1]锦州医学院附属第一医院心内科,辽宁锦州121000

出　　处：《心脏杂志》2006年第5期506-508,共3页Chinese Heart Journal

摘　　要：目的观察灯盏花素(BRE)对大鼠心肌缺血再灌注(I/R)诱导细胞凋亡及凋亡相关基因Bcl-2、Bax表达的影响,并对其作用机制进行初步探讨。方法30只SD大鼠随机分为3组(n=10):①假手术(Sham)组:前降支穿线不结扎,观察1.5 h;②缺血再灌注(I/R)组:结扎前降支30 m in,再灌注前5 m in生理盐水(4 mg/kg)iv,再灌注1h;③BRE组:再灌注前5 m in应用BRE(4 mg/kg)iv,余同I/R组。常规方法检测丙二醛(MDA)含量、超氧化物歧化酶(SOD)活性,免疫组化法测定Bc l-2、Bax蛋白的表达,通过细胞图像分析系统测量细胞阳性染色面积百分率,应用透射电镜观察心肌超微结构的变化。结果同I/R组相比,BRE组心肌组织MDA含量显著降低[(7.56±1.67)μmol/gvs(16.58±2.59)μmol/g,P<0.05],SOD活性显著升高[(2.37±0.22)mkat/gvs1.38±0.14 mkat/g,P<0.05],Bax蛋白表达显著降低(5.02±1.01vs18.59±5.68,P<0.01),而Bc l-2蛋白表达显著增高(17.48±4.72vs7.32±2.05,P<0.01),Bax/Bc l-2显著降低(0.29±0.57vs3.32±0.10,P<0.01),细胞阳性染色面积百分率显著减少(0.42±0.06vs13.84±1.97,P<0.01),细胞超微结构损伤明显减轻。结论BRE通过调控Bax/Bc l-2表达抑制心肌I/R诱导的细胞凋亡,对缺血再灌注心肌具有保护作用。AIM To observe the effect of Breviscapine on myocyte apoptosis and expression of Bcl-2 and Bax in myocardial ischemia-reperfusion rat model and to investigate the preliminary mechanism of Breviscapine. METHODS Thirty Sprague-Dawley rats were randomly divided into three groups （ n = 10 ） ： ①Sham operation group;②Ischemia-reperfusion （I/R） group, in which the left coronary artery was occluded for 30 mix followed by 1 hour reperfusion, and normal saline （4mg/kg） was given intravenously 5 mix before the initiation of reperfusion; ③ Breviscapine group, in which treatment similar to that of I/R group was given except in place of normal saline Breviscapine （4 mg/kg） was given intravenously 5 mix before the initiation of reperfusion. Routine method was used to detect the activity of superoxide dismutase （SOD） and the content of malondialdehyde （MDA）. The expressions of Bcl-2 and Ban protein were measured by immunohistochemical technique. Image processing system was used to quantitatively dispose the positive metric substance of immunohistochemistry. The uhrastucutural change of the myocardium was observed under electorn microscope. RESULTS Compared with those in I/R group, the myocardial MDA contents decreased significantly [ （7.56 ± 1.67 ） μmol/g vs （ 16.58 ± 2.59 ） μmol/g, P 〈 0.05 ] while the myocardial SOD activities increased significantly in Breviscapine group [ （2.37 ± 0.22） mkat/g vs （1.38 ±0.14）mkat/g, P 〈0.05 ]. In Breviscapine group, the protein expression of Bax gene obviously decreased （ 5.02 ± 1. 01 vs 18.59 ± 5.68, P 〈0.01 ）, the protein expression of Bcl-2 gene obviously inereased （ 17.48± 4.72 vs 7.32 ± 2.05, P 〈 0. 01 ）, the number of Bax/Bcl-2 was obviously decreased （0.29 ± 0.57 vs 3.32 ± 0.10, P 〈 0.01 ）, and the positive metric substance of immunohistochemistry decreased markedly （0.42 ±0. 06 vs 13.84 ± 1.97, P 〈0.01 ）. The pathological changes of myocardial uhrastnneture in Breviscapine group showed relativel

关 键 词：灯盏花素 心肌再灌注损伤 细胞凋亡 基因表达 

分 类 号：R541.4[医药卫生—心血管疾病]