低频电针对海洛因觅药行为及相关脑区FosB蛋白表达的影响  

Effects of electroacupuncture of low frequency on heroin-seeking behavior and FosB protein expression in relative brain regions

作　　者：孙丽敏[1] 甄利波[1] 刘宜军[1] 刘惠芬[2] 张富强[2] 杨国栋[2] 周文华[2] 

机构地区：[1]上海中医药大学,上海201203 [2]宁波戒毒研究中心

出　　处：《中国针灸》2006年第11期833-837,共5页Chinese Acupuncture & Moxibustion

基　　金：国家重大基础研究项目:2003CB5154;国家自然科学基金资助:30470554

摘　　要：目的:观察低频电针对海洛因觅药行为和FosB蛋白在相关脑区表达的影响,探讨电针作用机制。方法:用累进固定比率程序建立大鼠海洛因复吸模型,动物随机分3组:“三阴交”留针对照组、低频弱电针组和低频强电针组。用条件性线索和小剂量海洛因引燃诱导海洛因觅药行为;运用免疫组化技术观察FosB蛋白表达。结果:与治疗前比较,留针对照组和弱电针组均能减少海洛因戒断大鼠条件性线索诱导的觅药行为,弱电针组处理后小剂量海洛因引燃诱导的觅药行为也较对照组明显减少,同时,弱电针组处理后伏隔核、腹侧苍白球、基底外侧杏仁核等脑区FosB蛋白的表达明显减少。而强电针组对觅药行为虽有一定抑制作用,但无统计学意义,海洛因引燃后活动度较对照组和弱电针组增高。结论:低频电针低强度刺激能治疗海洛因觅药行为,这种作用可能与调节伏隔核、基底外侧杏仁核等脑区的神经适应有关。Objective To observe effects of electroacupuncture （EA） of low frequency on heroin-seeking behavior and FosB protein expression in relative brain regions so as to explore the mechanism of EA. Methods Rat model of relapsing into heroin was established with progressive fixed ratio program, and model rats were randomly divided into 3 groups： a ＂Sanyinjiao＂ needle-retention control group, a low frequency and weak EA group, and a low frequency and strong EA group. Heroin-seeking behavior was elicited by conditional clue and small dose of heroin; FosB protein expression was investigated with immunohistochemical technique. Results After treatment, the heroin-seeking behavior induced by conditional clue decreased in the needle-retention control group and the weak EA group, and the heroin-seeking behavior induced by small dose of heroin in the weak EA group significantly reduced as compared with the control group, and FosB protein expression in the nucleus accumbens septi, globus pallidus, basolateral amygdaloid nucleus significantly decreased in the weak EA group, and did not significantly change in the strong EA group; the activity induced by heroin increased as compared with those in the control group and the weak EA group. Conclusion EA of low frequency and low intensity can cure the heroin-seeking behavior, which is correlated with regulating nervous adaptation of nucleus accumbens septi, basolateral amygdaloid nucleus, etc..

关 键 词：海洛因依赖/针灸疗法 电针 基因 FOS 基因表达 

分 类 号：R245.3[医药卫生—针灸推拿学]