5-氮胞苷对籼稻成熟胚愈伤组织生长和再分化的影响及诱变效应  

EFFECTS OF 5-AZACYTIDINE ON THE PROLIFERATION,REGENERATION AND MUTATIONINDUEING TO THE CALLUS FROM INDICA RICE MATURE EMBRYO

作　　者：刘传光[1] 涂从勇 江奕君[1] 高云[1] 刘军[1] 林青山[1] 

机构地区：[1]广东省农业科学院水稻研究所,广东广州510640

出　　处：《核农学报》2006年第5期353-357,共5页Journal of Nuclear Agricultural Sciences

基　　金：国家农业科技跨越计划"中国超级稻育种技术及生产技术集成"(99-01-02)

摘　　要：用5-氮胞苷（5-azaC）处理籼型三系杂交水稻恢复系“明恢63”、“密阳46”和“特青2号”成熟胚诱导产生的愈伤组织，当5-azaC浓度为0．1mmol／L时，愈伤组织的增殖明显受抑；当5-azaC浓度达1．0mmol／L时，愈伤组织增殖率下降50％以上；5-azaC浓度提高到1．5mmol／L时，愈伤组织增殖率仅及对照的20％。分化阶段愈伤组织对5-azaC更敏感，当5-azaC的浓度为0．02mmol／L时，愈伤组织的再分化率明显下降，且随5-azaC浓度的增加，再分化率也显著下降，当5-azaC的浓度为0．2mmol／L时，各试验品种的愈伤组织的再分化力完全受抑，不能分化出幼苗。经5-azaC处理的愈伤组织再分化力也下降，但经预分化培养后分化力可恢复到较高水平。经5-azaC处理的愈伤组织再生植株产生了性状变异，出现了白化苗、矮秆、迟熟和早熟等变异体，当代的突变率为3．03％～5．31％。突变性状表现为单基因隐性突变，可稳定遗传。The callus, which were induced from the mature embryo of three-line indica hybrid rice restorer lines, Minghui 63, Milyang 46, and Teqing 2, were treated by 5-azacytidine （5-azaC）. The callus was inhibited obviously on the medium containing 0.1 mmol/L 5-azaC, and more obviously on the higher concentration of 5-azaC. The proliferation rate of the callus fall down over 50% when 5-azaC concentration was up to 1.0mmol/L. When the callus was exposed on the medium containing 1.5 mmol/L 5-azacytidine, its proliferation rate fall down over 80% . The callus was more susceptible to 5-azaC in the stage of regeneration. Compared with no 5-azacytidine in the medium, the regeneration rate of the callus fall down obviously when the callus were on the medium containing 0.02mmol/L 5-azaC. With higher concentration of 5-azaC, the regeneration rate of the callus was lower. The regeneration capability of the callus from three varieties tested was inhibited completely when the 5-azaC concentration was up to 0.2mmol/L and no plantlet was produced. The regeneration capability of the callus also fall down after the 5-azaC treatment, however, it could be restored through pre-regeneration stage. In this research system, several characteristics such as albino, dwarfism, late-heading and early-heading in the regeneration plants were mutated, the general mutation rate in MRI were 3.03 % -5.31%. The mutant characteristics appeared just single locus mutation and inheritable stably.

关 键 词：5-氮胞苷 籼稻 愈伤组织 再分化 诱变 

分 类 号：S511.22[农业科学—作物学]