OPS法玻璃化冷冻兔胚胎的研究  

Study on Vitrification of Rabbit Embryos with the Open Pulled Straw(OPS) Method

作　　者：丁志丽[1] 周俊波[2] 戴荣亮[2] 王杏龙[2] 

机构地区：[1]湖州师范学院生命科学学院,浙江湖州313000 [2]扬州大学动物科学与技术学院,江苏扬州225009

出　　处：《中国畜牧杂志》2006年第19期12-14,共3页Chinese Journal of Animal Science

基　　金：江苏省教育厅(02KJD230001);湖州师范学院校级课题(KX23031)

摘　　要：采用2种不同浓度的冷冻液(VSⅠ和VSⅡ),通过OPS法玻璃化冷冻兔的胚胎。结果表明:冷冻兔的桑椹胚641枚,解冻后胚胎总回收率为93.8%(601/641),形态结构良好率为94.5%(568/601),透明带完好率为100%(601/601)。VSⅠ和VSⅡ冷冻的胚胎解冻后回收率分别为93.8%(305/325)和93.7%(296/316),两者差异不显著(P>0.05);形态良好率分别为95.1%(290/305)和94.0%(278/296),前者显著地高于后者(P<0.05)。将解冻后形态结构良好的桑椹胚415枚移植到29只受体母兔体内,总妊娠率为51.7%(15/29),产仔率为34.5%(61/177)。VSⅠ妊娠率为53.3%(8/15),产仔率为36.8%(35/95);VSⅡ妊娠率为50.0%(7/14),产仔率为31.7%(26/82)。无论妊娠率还是产仔率,VSⅠ都高于VSⅡ,但差异不显著(P>0.05)。Rabbit embryos were vitrified with two different concentration freezing media （VSⅠ and VS Ⅱ ） with the OPS method. The results showed （1） 641 morulas were vitrified. After thawing, the overall embryos＇ recovery rate was 93.8% （601/641）, 568 of which （94.5%） were morphologically intact embryos, and all of 601 embryos＇ zona pellucid were intact （100%）. The embryos＇ recovery rate of VS Ⅰ and VS Ⅱ were 93.8% （305/325） and 93.7% （296/316） respectively. 290 （95.1%） and 278 （94.0%） were morphologically intact embryos for VS Ⅰ and VS Ⅱ, respectively. There was no difference in the embryos＇ recovery rate between VS Ⅰ and VS Ⅱ, and the rate of morphologically intact embryos for VS Ⅰ was remarkably higher than that for VS Ⅱ （P 〈 0.05）. （2） After thawing, 415 morulas of good quality were transferred into 29 recipients. The overall pregnant rate was 51.7%（15/29） and the rate of embryos developing into young pups was 34.5%（61/177）. The pregnant rate and the rate of embryos developing into young pups for VS Ⅰ were 53.3% （8/15） and 36.8% （35/95）, respectively. The correspondent values for VS Ⅱ were 50.0%（7/14） and 31.7% （26/82）. The values of VS Ⅰ were higher than that of VS Ⅱ, but the difference was not significant（P 〉 0.05）.

关 键 词：畜牧学 OPS 胚胎冷冻 兔 

分 类 号：S829.103[农业科学—畜牧学]