大鼠门静脉阻断对肠黏膜屏障影响的实验研究  

Change of Intestinal Mucosa Barrier after Occlusion of Portal Vein in Rats

作　　者：徐敬[1] 王彦坤[1] 王晓云[1] 沈骅[2] 莫一我[1] 

机构地区：[1]云南省第一人民医院肝胆外科,云南昆明650014 [2]昆明市第一人民医院检验科,云南昆明650011

出　　处：《中国误诊学杂志》2006年第21期4114-4116,共3页Chinese Journal of Misdiagnostics

摘　　要：目的:探讨阻断大鼠门静脉对肠屏障功能的影响,为临床门静脉阻断后肠屏障功能的保护提供实验依据。方法:SD大鼠70只,随机分为对照组、手术组。手术组门静脉阻断时间分别为30、40、60 m in,门静脉复流4 h。70只动物均于手术后5 h处死,无菌条件下取门静脉血2 m l检测内毒素;取回盲部肠系膜淋巴结作细菌培养;另在距回盲部2 cm处取小肠肠壁组织1 cm,作病理学检查。结果:对照组与手术组比较,大鼠门静脉阻断后其门静脉血清内毒素水平明显上升;肠系膜淋巴结细菌培养阳性率增加;小肠壁形态学结构亦发生明显改变。上述改变随门静脉阻断时间的延长而更加明显。结论:大鼠门静脉阻断后可导致其肠屏障功能的损害,其损害程度与门静脉阻断时间呈正相关。Objective：To study the effect of portal vein occlusion on intestinal mucosa barrier in rats, to present the experimental data for the clinical surgery. Methods ： 70 Sprague-Dawley rats were randomly divided into controlled group,operation group. The portal vein were occlused 30,40,60 min respectively in the operation groups. All the rats were killed 5 hours after operation. And 2ml blood from portal vein,lymph nodes around appendix, 1 cm small intestine wall were taken for endotoxin levels,bacterial translocation and pathiology examinations. Results：Compared between the control group and the operation group ,endotoxin levels, bacterial translocation rates rised greatly and gut structure change obviously in the later. The above change became more obvious with the increase of portal vein occlusion time. Conclusion：The occlusion of portal vein can lead the decrease of intestine mocusa barrier and the increase of its permeability. There is a positive correlation between teabove change and the time of portal vein occlusion.

关 键 词：门静脉/外科学 肠粘膜/病理生理学 

分 类 号：R657.3[医药卫生—外科学]