理气化痰祛瘀法对非酒精性脂肪性肝炎大鼠纤溶酶原激活物及其抑制物的影响  

Effects of the Method for Regulating Qi to Dissipate Phlegm and Blood Stasis on Plasminogen Activator and Its Inhibitor in Non-Alcoholic Steatohepatitis Rats

作　　者：严茂祥 陈芝芸 施军平 刘庆生 何蓓晖 

机构地区：[1]浙江中医学院附属医院消化病研究室,杭州市邮电路54号310006

出　　处：《中医杂志》2006年第10期775-777,共3页Journal of Traditional Chinese Medicine

基　　金：浙江省教育厅资助项目(20020080)

摘　　要：目的:观察理气化痰祛瘀中药对高脂饮食诱导的非酒精性脂肪性肝炎(NASH)大鼠血浆组织纤溶酶原激活物(t-PA)和纤溶酶原激活物抑制物(PAI-1)活性的影响,探讨其防治NASH的作用机理。方法:以高脂饮食喂养12周诱导Wistar大鼠建立NASH模型,以不同剂量的理气化痰祛瘀中药预防及治疗,光镜观察肝组织切片病理学改变,发色底物法测定血浆t-PA、PAI-1水平。结果:NASH模型大鼠肝组织出现了严重脂肪变和不同程度的炎症细胞浸润、肝细胞坏死、汇管区渗出;血浆PAI-1活性和PAI-1/t-PA值较正常大鼠显著升高;应用理气化痰祛瘀中药进行预防和治疗后,大鼠肝组织脂肪变程度和炎症活动程度显著改善,同时血浆PAI-1活性和PAI-1/t-PA值显著降低。结论:PAI-1可能参与高脂饮食诱导的大鼠NASH的发生发展,理气化痰祛瘀中药能调节PAI-1的分泌和纤溶系统平衡,防止NASH的进一步发展。Objective：To investigate effects of the method for regulating qi to dissipate phlegm and blood stasis on plasma tissue-type plasminogen activator （t-PA） and plasminogen activator inhibitor （PAI-1） in non-alcoholic steatohepatitis （NASH） rats, and to explore the prevention and treatment mechanism of NASH. Methods.-NASH model rat was induced by 12-week high fat diet ,and different doses of Chinese herbs for regulating qi to dissipate phlegm and blood stasis were given to prevent and treat NASH. Plasma t-PA and PAI-1 were detected with chromophorous substrate assay, and the pathological changes of the liver were observed with optical microscope. Results： The liver tissue of NASH rats showed severe steatosis, and different degrees of inflammation,cell infiltration,necrosis of hepatocytes and exudation in the portal area as compared with the normal rats ; Plasma PAI-1 activity and the ratio of PAI-1/t-PA in the model group were significantly increased. Chinese herbs could improve the steatosis of hepatocytes and inflammatory activity,and significantly decrease the levels of plasma PAI-1 activity and the ratio of PAI-1/t-PA. Conclusion：PAI-1 possibly participates in the generation and development of NASH induced by high fat diet,and Chinese herbs for regulating qi to dissipate phlegm and blood stasis can significantly regulate the secretion of PAI-1, and the balance of the fibrinolytic system,and can prevent further development of NASH.

关 键 词：脂肪肝 非酒精性/中医药疗法 @理气化痰祛瘀 

分 类 号：R259[医药卫生—中西医结合]