壳聚糖混合膜酶降解的FTIR分析  

FTIR Analysis of the Enzymatic Degradation of Chitosan Blend Membranes

作　　者：任东文[1] 王一力[1] 包德才[1] 谢威扬[1] 马小军[1] 

机构地区：[1]中国科学院大连化学物理研究所生物医学材料工程组中国科学院研究生院,辽宁大连116023

出　　处：《光谱学与光谱分析》2006年第10期1825-1828,共4页Spectroscopy and Spectral Analysis

基　　金：国家自然科学基金项目(2002CB713804);国家"863"计划项目(2001AA326020)资助

摘　　要：生物可降解性是壳聚糖的重要性质之一,但利用傅里叶变换红外光谱(FTIR)研究降解过程中壳聚糖的变化则较少。文章制备了由高脱乙酰度壳聚糖(HDC)和中脱乙酰度壳聚糖(MDC)组成的混合膜。运用FTIR分析了壳聚糖混合膜组分变化对其红外谱图和脱乙酰度(DD)的影响,并研究了该混合膜在溶菌酶的降解作用下红外谱图和脱乙酰度的变化。发现壳聚糖混合膜材料的脱乙酰度与膜中MDC组分的比例呈线性关系;随降解的进行,混合膜的脱乙酰度增加。结果证实了溶菌酶对较低脱乙酰度壳聚糖的选择性降解作用,而且表明FTIR可用于分析壳聚糖混合膜降解过程中的化学变化。Chitosan blend membranes were prepared by casting the mixture solution of highly deacetylated chitosan （HDC） and moderately deacetylated chitosan （MDC）. FTIR was used to investigate the components of the blend membranes before and after lysozymic degradation. It was found that the ratio of MDC in the blend membranes had a linear relation with the degree of deacetylation（DD） of the membranes. In enzymatic degradation process, DD of the membranes exhibited an increasing tendency. The F-FIR data indicate that MDC component in the blend membranes can be removed by selective enzymatic degradation. This also suggests that FTIR can be used as an efficient and rapid method to investigate the degradation process of chitosan.

关 键 词：壳聚糖 膜 溶菌酶 降解 红外光谱 

分 类 号：O657.3[理学—分析化学]