光谱法研究甲氨喋呤与牛血清白蛋白的相互作用  

Study of the Interaction between Methotrexate and Bovine Serum Albumin by Spectrometry

作　　者：谢文俊[1] 冯玉萍[1] 曹胜利[2] 赵玉芬[1] 

机构地区：[1]清华大学化学系生命有机磷化学及化学生物学教育部重点实验室,北京100084 [2]首都师范大学化学系,北京100037

出　　处：《光谱学与光谱分析》2006年第10期1876-1879,共4页Spectroscopy and Spectral Analysis

基　　金：北京市自然科学基金(7042006)资助项目

摘　　要：用荧光和紫外吸收光谱法,研究了抗癌药物甲氨喋呤(MTX)与牛血清白蛋白(BSA)的相互作用。结果表明:在生理条件下,甲氨喋呤对牛血清白蛋白荧光有较强的猝灭作用,其猝灭方式为静态猝灭。根据猝灭结果,求出了不同温度下反应的结合常数及反应热力学参数,并据此确定了它们相互作用的主要形式。The interaction of methotrexate （MTX） and bovine serum albumin （BSA） was stuciled by tiuorescence ana adsorption spectra. The results show that methotrexate strongly quenched the fluorescence of bovine serum albumin, and the quenching mechanism is a static quenching procedure. The binding constants and the number of binding sites between MTX and BSA at different temperatures were calculated. Furthermore, the enthalpy and entropy changes in the interaction were also obtained, and the primary binding pattern between MTX and BSA was interpreted as hydrophobic interaction.

关 键 词：甲氨喋呤 牛血清白蛋白 荧光光谱 紫外光谱 相互作用 

分 类 号：O657.3[理学—分析化学]