果糖二磷酸锶盐对D-半乳糖致小鼠睾丸退行性病变的改善  

Prophylaxis of strontium fructose 1,6-diphosphate(FDP-Sr) ameliorates for the D-galactose-induced testis degeneration in mice

作　　者：刘浩然[1] 张琪[2] 戴德哉[1] 应汉杰[2] 戴茵[1] 

机构地区：[1]中国药科大学药理研究室,南京210009 [2]南京工业大学制药与生命科学学院,南京210009

出　　处：《中国新药杂志》2006年第20期1735-1738,共4页Chinese Journal of New Drugs

基　　金：国家自然科学基金(30572217)

摘　　要：目的:研究果糖二磷酸锶盐(FDP-Sr)对D-半乳糖致雄性小鼠睾丸退行性病变的影响。方法:昆明种雄性小鼠皮下注射D-半乳糖200 mg·kg-1连续30 d造成睾丸退行性病变。同时分别灌胃给予FDP-Sr 50,100,200 mg·kg-1及阳性对照药物生力雄丸853 mg(生药)·kg-1,qd,连续30 d。观察各组小鼠的扑提性行为(扑捉潜伏期和20 min内雄鼠扑捉雌鼠的次数),检测血清睾酮、睾丸组织特异性酶类及病理学变化。结果:与正常对照组比较,D-半乳糖模型组小鼠扑捉潜伏期延长,扑捉次数减少;睾丸、附睾、精囊腺、包皮腺重量指数下降;血清睾酮下降,睾丸中琥珀酸脱氢酶(SDH)、乳酸脱氢酶(LDH)、酸性磷酸酶(ACP)及谷氨酰转肽酶(γ-GT)活力降低;病理学检测可见生精上皮层变薄,各级生精细胞减少。FDP-Sr 100和200 mg·kg-1组上述现象明显改善(P<0．05),其中FDP-Sr 200 mg·kg-1组与生力雄丸组(853 mg·kg-1)效果相当。结论:FDP-Sr对D-半乳糖致雄性小鼠睾丸退行性病变有明显的改善作用,且呈剂量依赖关系。Objective ：To investigate the effect of strontium fructose-1,6-diphosphate（FDP-Sr） for D-galactose-induced testis degeneration in mice. Methods：The Kunming male mice were subcutaneously injected with D-galactose 200 mg·kg^-1 for 30 days to induce the testis degeneration. Simultaneously, the mice were i.g. administered with FDP-Sr（50,100 or 200 mmg·kg^-1 ） or Shenglixiong pills（853 mg·kg^-1 ） for 30 days. The sexual behavior was evaluated based on the mating latent period and mating frequency of the male mice to female mice within 20 minutes. The testosterone level in serum and the activities of succinodehydrogenase （ SDH ） , lactate dehydrogenase （ LDH ） , acid phosphatase （ACP） and γ-glutamyltranspeptidase（γ-GT） in the testis and the histopathological changes were monitored. Results：Compared to the normal control, the D-galactose-induced mice showed prolonged mating latent period, less mating frequency and reduced weight indexes of testis, epididymis, seminal vesicle and foreskin gland. The mice experienced decreases of testosterone level in the serum and activities of SDH, LDH, ACP and γ- GT in the testis. The histopathological examination suggested that seminiferous tubule diameters and various grade of spermatocytes in the testis markedly shrunk. The FDP-treated mice at the doses of 100 and 200 mg·kg^-1 showed significant improvements of the dysfunctional symptoms （ P 〈 0.05 ）. FDP-Sr 200 mg·kg^-1 was therapeutically equivalent to Shenglixiong pills（853 mg·kg^-1 ）. Conclusion ： FDP-Sr has a prophylaxis of the mice testis degeneration in a dose-dependent manner.

关 键 词：果糖二磷酸锶盐 D-半乳糖 睾丸 退行性病变 

分 类 号：R965.1[医药卫生—药理学] R339.38[医药卫生—药学]