环氧化酶-2在新生大鼠缺氧缺血性脑损伤的表达  

The expression of cyclooxygenase-2 in neonatal rat with hypoxic-ischemic brain damage

作　　者：张萍[1] 李冬[1] 曲云霞[1] 李海燕[2] 

机构地区：[1]大连医科大学附属第二医院儿科,116027 [2]鞍山市中心医院儿科

出　　处：《中国新生儿科杂志》2006年第5期275-277,共3页Chinese Journal of Neonatology

摘　　要：目的探讨环氧化酶-2(COX-2)在新生儿缺氧缺血性脑损伤(HIBD)中的作用。方法新生7日龄大鼠制成HIBD模型,缺氧时间为2h,RT-PCR半定量分析COX-2mRNA,免疫组化方法测定COX-2蛋白表达,光镜下观察神经元坏死情况。结果HIBD后6、24、48h,5dCOX-2mRNA表达出现不同程度的表达增强,分别为(0·461±0·052),(0·887±0·0816),(0·660±0·119),(0·474±0·104),与对照组(0·321±0·175)比较,差异有统计学意义(P<0·01),其中HIBD24hCOX-2mRNA表达为最高峰,同其余各组相比,差异均有统计学意义(P<0·01),免疫组化结果与之一致。结论COX-2在新生儿HIBD形成中发挥一定的作用。Objective To study the role of cyclooxygenase-2 （COX-2） in neonatal rat with hypoxic-ischemic brain damage （HIBD）. Methods With the neonatal rat model of HIBD, hypoxia time was 2 hours, the expression of COX-2 mRNA was analyzed by RT-PCR, and COX-2 protein expression was studied by immunohistochemistry. The expression of COX-2 mRNA and COX-2 protein were studied at 6, 24, 48 hours and 5 clays after hypoxia. Results COX-2mRNA expression increased after HIBD, （0. 461 ±0. 052）, （0. 887±0. 0816）, （0. 660 ± 0. 119）, （0. 474 ±0. 104 ） respectively, The difference was significant compared with control group（0. 321±0. 175）, P 〈0. 01. The peak appeared at 24 hours after HIBD, and it was higher than other time points. Similar time course of COX-2 protein expression was seen, （42. 50 ± 75, 88 ）, （ 837. 50 ± 88.84）, （ 510. 00 ± 49.67 ）, （ 242. 50 ± 47.89 ）. Compared with control group, （21.75 ± 12. 84）, P 〈 0. 01. Conclusions COX-2 may play a role in neonatal HIBD development.

关 键 词：酶类 缺氧缺血 脑 大鼠 

分 类 号：R722.1[医药卫生—儿科]