γ-氨酪酸治疗新生大鼠缺氧缺血性脑损伤的实验研究  

Therapeutic effect of γ-aminobutyric acid on neonatal rat hypoxic-ischemic brain damage

作　　者：郑华 杨波[2] 周品山 李世芳 

机构地区：[1]山东省东营市人民医院儿科,257012 [2]山东省立医院儿科

出　　处：《中国新生儿科杂志》2006年第5期278-281,共4页Chinese Journal of Neonatology

摘　　要：目的探讨γ-氨酪酸对新生大鼠缺氧缺血性脑损伤(HIBD)的治疗作用。方法采用Rice法制备新生大鼠HIBD模型,用γ-氨酪酸(GABA)灌服HIBD大鼠,用TUNEL法监测大鼠脑组织切片各时间点的细胞凋亡数,用化学方法检测各时间点血清中的超氧化物歧化酶(SOD),一氧化氮(NO),诱生型一氧化氮合酶(iNOS)的浓度。结果(1)缺氧缺血(HI)组各时间点的细胞凋亡率均明显高于假手术对照组;72h时间点的细胞凋亡率最高。(2)GABA治疗组与HI组相比,各时间点的凋亡率均明显降低、SOD值明显升高。(3)发病早期6h时间点HI、GABA两组与正常对照(N)组NO、iNOS浓度比较,差异无统计学意义。两组其余各时间点的NO、iNOS值均明显高于假手术正常对照组,HI、GABA两组比较,GABA各时间点的值均明显低于HI组。结论应用γ-氨酪酸治疗HIBD大鼠,可明显降低HIBD新生大鼠的脑细胞凋亡率。Objective To observe the therapeutic effect of γ-aminobutyric acid （GABA）on neonatal rat hypoxic-ischemia brain damage （HIBD）. Methods Neonatal rat HIBD model was made with Rice method. GABA （0. 25 mg/g Cerebral cortex apoptosis was studied by by chromatometry at 6, 24, 72 h and 5 d higher percentage of apoptosis appeared Qd） was given by gavage to study group （GABA group ）. TUNEL method, and serum SOD, NO, iNOS were determined after HIBD. Results Compared with control group, these was in HIBD group, and its peak was noted at 72 h after HIBD. GABA groups had lower percentage of apoptosis and higher SOD level than HIBD group. Both HIBD group and GABA group had higher NO, iNOS level 6 h later after HIBD, but the level was lower in GABA group in whole study course. Conclusions Neonatal rat HIBD increases cerebral cortex apoptosis and NO serum level. GABA application can increase SOD activity, inhibit iNOS activity, and reduce the NO concentration. GABA supply can help to reduce cerebral cortex apoptosis.

关 键 词：GABA调节剂 大鼠 缺氧缺血 脑 

分 类 号：R722.1[医药卫生—儿科]