^(125)I-IL-2在自身免疫胰岛炎NOD小鼠中核素显像应用的探讨  

Distribution of ^(125)Iodine-labeled Interleukin-2 and its relation to insulitis in NOD mice

作　　者：邝建[1] 周智广[2] 刘长征[3] 杨华章[1] 梁昌盛[3] 潘甜美[4] 

机构地区：[1]广东省人民医院内分泌科,广州510080 [2]中南大学湘雅二医院代谢内分泌研究所 [3]中山大学医学部实验核医学教研室 [4]广东省医学实验动物中心

出　　处：《中国糖尿病杂志》2006年第5期377-379,共3页Chinese Journal of Diabetes

基　　金：广东省科技计划资助项目(2003C30503)

摘　　要：目的探讨IL-2作为自身免疫胰岛炎核素显像药物的可行性。方法将38只雌性未发病NOD小鼠和26只Balb/c小鼠随机分为5个时间组,尾静脉注射酶法标记的125I-IL-2,相应时间处死后测定血液和腹腔各脏器放射性。另3只NOD小鼠在注射131I-IL-230min后行SPECT-CT仪扫描显像。结果注射后10至120min,NOD小鼠胰腺125I-IL2聚集性明显高于Balb/c小鼠,且胰腺放射性与胰岛炎程度呈线性正相关(R2=0.614~0.776,P<0.05);但胰腺/周围组织放射性比值普遍较低;去除脾脏、肝脏和双肾,NOD小鼠胰腺方可显像。结论125I-IL-2在自身免疫胰岛炎的NOD小鼠胰腺有相对高聚集性,但有较高放射性背景,影响局部显像效果。Objective To evaluate the feasibility of ^125Iodine -labeled IL-2 scintigraphic methods for the imaging of lymphocyte infiltration of the Langerhans＇ islets. Methods ^125I-IL2 was injected intravenously to pre-diabetic female NOD mice （aged 16-17 weeks）and female Balb/c mice as control. Animals were sacrificed at different time points and the radioactivity in different organs was measured. ^125I-IL-2 was injected iv to 3 pre-diabetic female NOD mice, and pancreas was imaged through SPECT after 30 minutes injection. Results The mean radio activities in the pancreas of NOD mice were significantly higher as compared to those of Balb/c mice at time points from 30 to 120 minutes （P （0. 001）, and it was positively correlated with insulitis score （R^2 =0. 614-0. 776, P〈0. 03）. The uptake ratios of pancreas over surrounding organs were almost all lower than 2. 0. Pancreas was imaged only 30 minutes after ^125I-IL-2 injection while spleen, liver and kidney were ablated. Conclusions The preliminary data show that the ^125Iodine labeled IL-2 accumulates significantly in the pancreas of NOD mice, but it is needed to eliminate the higher radioactivity background in order to visualize the pancreas imaging.

关 键 词：胰岛炎 白介素-2 125I碘 NOD小鼠 核素显像 

分 类 号：R587.1[医药卫生—内分泌]