护肾固精方对大鼠系膜细胞增殖周期的影响  

Effect of Hushengujingfang on the Proliferation of Rat Mesangial Cells

作　　者：周清发[1] 高杨[1] 武宏艳[1] 范一超[1] 宋书贤[1] 雷宏强[1] 徐薇[1] 

机构地区：[1]西安交通大学第二医院,陕西西安710004

出　　处：《中国中医药信息杂志》2006年第11期35-36,共2页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：国家自然科学基金资助项目(30271623);陕西省中医药管理局资助项目(046)

摘　　要：目的探讨中药复方护肾固精方对大鼠系膜细胞(MC)生物学行为的影响。方法采用体外培养大鼠MC和血清药理学方法,分为护肾固精方血清组、脂多糖(LPS)病理组及正常对照组,将不同浓度的作用物加入MC共同培养,培养后的MC制成单细胞悬液,经流式细胞仪检测后,计算出细胞周期中的G0/G1期、S期、G2/M期各时期的百分比及S期抑制百分比、细胞PI。结果不同浓度的作用物对MC增殖周期有不同影响。LPS血清能明显刺激MC增殖,表现为G0/G1期细胞百分比减以及S期细胞增加;细胞的S期抑制百分比明显下降,而细胞的PI则显著增高;中、高浓度的中药血清明显抑制MC增殖,表现为G0/G1期细胞百分比增加及S期细胞减以,细胞的S期抑制百分比明显增加,而细胞的PI则显著降低。并且使大量MC阻滞在G0/G1期,能诱导MC发生凋亡。结论护肾固精方治疗系膜增殖性肾炎等肾小球疾病的作用机理之一,可能与抑制MC增殖和促其细胞凋亡有关。Objective To study the effect of the Hushengujingfang （HSGJF）, a traditional Chinese prescription, on the biological behaviour of rat mesangial cell （MC）. Methods MC was cultured in vitro, and the serologic pharmacology method was employed. The ceils were divided into serum groups of HSGJF, LPS group and control group. Added the different concentration actors to MC and cocultured then made into urdceU suspension. After examinning it by flow cytometry, the percentage of G0/G1 period, S period, G2/M period in cell cycle and the inhibition percentage of S period and cell proliferation index were calculated. Result The actors of different concentration had different effects to the multiplication cycle of MC and delayed the entry of cell cycle at G0/G1 period. HSGJF induced apoptosis of MC. Conclusion One of action mechanism of HSGJF treating mesangial proliferative nephritis and other renal glomerular diseases is to inhibit MC proliferation and promote apoptosis.

关 键 词：护肾固精方 系膜细胞 增殖周期 细胞凋亡 

分 类 号：R285.5[医药卫生—中药学]