RP-HPLC法测定温阳通痹丸中盐酸麻黄碱的含量  

Determination of the Content of Ephedrine Hydrochloride in Wenyang Tongbi Wan by RP-HPLC

作　　者：朱庄松[1] 林建昌[1] 王志梅 

机构地区：[1]梅州市人民医院,广东梅州514031 [2]梅州市药品检验所,广东梅州514021

出　　处：《中国中医药信息杂志》2006年第11期59-60,共2页Chinese Journal of Information on Traditional Chinese Medicine

摘　　要：目的建立以反相高效液相色谱法测定温阳通痹丸中盐酸麻黄碱含量的方法。方法色谱柱为DDS-C18柱(4.6mm×150mm,5μm);流动相为甲醇-水(1∶1),检测波长为254nm,柱温为30℃,流速为1mL/min。结果盐酸麻黄素进样量在1.3086～3.9180μg范围内与吸收峰面积呈良好的线性关系(r=0.9993,n=5),平均加样回收率98.91%,RSD=0.68%(n=5)。结论本方法简便、准确、重现性好,可用于温阳通痹丸的质量控制。Objective To establish a HPLC method for determination of the content of ephedrine hydrochloride in Wenyang Tongbi Wan. Methods A DDS-C18 colurnn （4.6 mm×150 mm, 5 μm） was used. The mobile phase was methane-water （1 ： 1）. The column temperature was 30 ℃. The flow rate was 1 mL/min. There was good linearity of ephedrine hydrochloride in the range of 1.3086～3.9180 μg （r=0-9993, n=5）. The average recovery was 98.91% （RSD=0.68%, n=5）. Conclusion This method is simple, accurate and repeatable, and can be used for quality control of Wenyang Tongbi Wan.

关 键 词：反相高效液相色谱法 温阳通痹丸 盐酸麻黄碱 含量测定 

分 类 号：R284.1[医药卫生—中药学]