葡萄幼胚培养技术的研究(Ⅱ)——接种时间及处理方法对胚培养的影响  

The Study on the Technique of Embryo Culture of Grape(Ⅱ)

作　　者：刘海琳[1] 尚忠海[1] 崔玲华[1] 廖康[2] 陈力耕[3] 

机构地区：[1]河南科技大学林业职业学院,河南洛阳471002 [2]新疆农业大学园艺学院,乌鲁木齐830052 [3]浙江大学农生学院果树研究所,杭州310029

出　　处：《河南科学》2006年第6期844-848,共5页Henan Science

基　　金：国家自然科学基金资助项目(30070634)

摘　　要：选用花后35￣60d的巨峰、里扎马特和无核白葡萄,取其幼胚接种于不同激素水平M S培养基上,进行胚培养,以研究接种时间及处理方法对胚培养的影响.结果表明对花后60d的里扎玛特和巨峰的幼胚进行胚培养,其发芽率和成苗率显著高于层积处理后催芽播种的种子的发芽率与出苗率.低温和横切处理能有效地促进葡萄幼胚的萌发.花后60d的巨峰和里扎马特的发育率和成苗率最高;花后60d的无核白的幼胚发育率最高,花后50d的幼胚成苗率最高.35-60 days after florescence, embryo of Kyoho（V. viniferax V. labrusce cv.）, Rizamat（V. vinifera cv.） and Thompson Seedless（V. vinifera cv. Suhanina） grapes were cultured on MS medium supplemented with different levels of concentrations of GA3, IAA and 6-BA in order to study the techniques of embryo rescue. The results indicated that the germination rate and seedling rate of the embryo culture were higher than those of insemination. The low temperature and the cutting treatments were affected to the young embryos germination. The highest development rate and germinating rate of Rizamat＇s and Kyoho＇s embryos were gotten by the time which was 60 days after florescence. The best period of inoculation of Thompson Seedless＇ embryos was 50 days after florescence.

关 键 词：胚培养 幼胚 接种时间 

分 类 号：S663.1[农业科学—果树学]