哮喘患者肺泡巨噬细胞中IL－6mRNA表达的研究  

Investigation of Interleukin-6 mRNA Expression in Asthmatic Alveolar Macrophages

作　　者：陈一强[1] 施焕中[1] 柳广南[1] 钟小宁[1] 梁国容[1] 许辉[1] 

机构地区：[1]广西医科大学一附院呼吸内科

出　　处：《广西科学》1996年第3期51-54,共4页Guangxi Sciences

摘　　要：应用逆转录酶链反应及索氏转移杂交技术分析过敏性支气管哮喘患者（激素治疗组９例，非激素治疗组１２例）及正常对照组（１０例）肺泡巨噬细胞（ａｌｖｅｏｌａｒｍａｃｒｏｐｈａｇｅｓ，ＡＭ）中白细胞介素一６（ｉｎｔｅｒｌｅｕｋｉｎ－６，ＩＬ－６）的ｍＲＮＡ表达。ＩＬ－６／β－ａｃｔｉｎ比值表示。结果发现未用激素治疗的哮喘患者ＡＭ的ＩＬ－６／μ－ａｃｔｉｎ为０．６６±０．３２，显著高于正常对照者（０．３４±０．１３，Ｐ＜０．０１）及激素治疗组（０．４１±０．１５，Ｐ＜０．０５）。提示表达ＩＬ－６的ＡＭ在哮喘的发病机理中可能具有重要的作用，而皮质激素治疗哮喘的机制之一可能与其抑制ＡＭ表达及产生ＩＬ－６的能力有关。The interleukin-6 (IL-6) mRNA expression was analyzed in alveolar macrophage (AM) from allergic asthmatics treated with corticosteriod (9 cases) or not (12 cases), as well as normal subjects (10 cases). This was achieved by using reverse transcription-polymerase chain reaction and Southern blots techniques. The result was described by IL-6/β-actin ratio and was found that the x ±s of IL-6/β-actin ratio in AM from asthmatics without corticosteriod treatment was 0. 66±0. 32, which significantly higher than that from either normal subjects (0. 34±0. 13, P <0. 01 ) or asthmatics treated with corticosteried (0. 41±0. 15, P < 0. 05 ). These results suggest that AM expression of IL-6 may be important in phathogenesis of human asthma, and that one respect of mechanisms of corticosteriod in asthma treatment may be inhibition of IL-6 and thus supression of inflammation in airway.

关 键 词：白细胞介素-6 基因表达 巨噬细胞 哮喘 肺泡 

分 类 号：R562.25[医药卫生—呼吸系统]