鸡志贺氏菌β-内酰胺酶的测定及其与耐药质粒的关系  

Detection of the β-Lactamase of S.flexneri from Chicken and Its Relation with the Resistant Plasmids

作　　者：梁军[1] 匡秀华[1] 苑丽[1] 胡功政[1] 杨玉荣[1] 司红彬[1] 

机构地区：[1]河南农业大学牧医工程学院,河南郑州450002

出　　处：《河南农业大学学报》2006年第5期510-515,共6页Journal of Henan Agricultural University

基　　金：国家自然科学基金资助项目(30471307)

摘　　要：通过对鸡志贺氏菌β-内酰胺酶及ESBLs的测定、质粒提取、细菌转化及质粒结合转移等试验,分析其耐药性与其质粒之间的关系,探讨耐药质粒在细菌间的传递方式.结果表明,质粒转化后的转化子对β-内酰胺类抗生素全部耐药,对氨基糖苷类、氟诺酮类有一定程度的敏感;而质粒接合传递体的耐药谱与质粒转化子一致.耐药菌株的质粒可编码β-内酰胺酶,且耐药质粒可通过结合转移方式传递至感受态大肠杆菌.This paper identifies the drug-resistance of S. flexneri related to resistant plasmids and detects the way of resistant plasmids transfering among the bacilli through detection of β-lactamase （indluding ES- BLs）and tests of S. flexneri resistant plasmids extraction, transformation and conjugation. The result shows： after resistant plasmids extraction and conversion, the recons are resistant to all β-lactam antibiotics but sensitive to quinolones and aminoglycosides to some degree; and the same as the zygotes. The plasmids of S. flexneri can code β-lactamase, and the resistant plasmids can be transferred to E. coli through conjugation and transformation.

关 键 词：志贺氏菌 超广谱Β-内酰胺酶 耐药质粒 质粒转化 接合传递 

分 类 号：S852.61[农业科学—基础兽医学]