不同浓度谷氨酸对海马神经元损伤及异丙酚的保护机制  

Effects of propofol on the injuried cultured hippocampal neurons with different dosage of glutamic acid

作　　者：年阿兴[1] 杨静[1] 李天佐[1] 张炳熙[1] 

机构地区：[1]首都医科大学附属北京同仁医院麻醉科,100730

出　　处：《北京医学》2006年第11期673-675,共3页Beijing Medical Journal

基　　金：北京市卫生重点扶植学科联合基金资助项目(编号99-06)

摘　　要：目的观察不同浓度谷氨酸对海马神经元的损伤及异丙酚对损伤的拮抗作用。方法培养12d的海马神经元细胞,随机分为4组:正常对照组(Control组)与含谷氨酸10μmol/L组(G10组)、50μmol/L组(G50组)及100μmol/L组(G100组)。再加入异丙酚500μmol/L再培养24h组(PG10、PG50、PG100组)。MTT法测定细胞存活率及免疫组化测定c-fos蛋白阳性面积率。结果与正常对照组相比,谷氨酸各组和异丙酚PG100组的细胞存活率下降(P<0.05),谷氨酸各组有剂量依赖性;与对应谷氨酸各组相比,异丙酚各组的细胞存活率上升(P<0.05)。位于胞核内的c-fos阳性蛋白在谷氨酸各组呈紫黑色且数量呈剂量依赖性增加(P<0.01),与对应谷氨酸各组相比,异丙酚各组能降低c-fos蛋白阳性面积率。结论外源性谷氨酸对海马神经元有呈剂量正相关的损伤作用,异丙酚能降低此损伤,减少c-fos蛋白过度表达,提高海马神经元的存活率。Objective To evaluate the effect of propofol on injuried primary cultured hippocampal neurons with different dosage of glutamic acid. Methods Hippocampal cell were cultured for 12 days and then randomly divided into three groups： control group, cultured with glutamic acid 10μmol/L,50μmol/L, 100μmol/L, for another 24 hours group and cultured with propofol and glutamic acid 10μmol/L,50μmol/L,100μmol/L. Cell survival ratio was assayed and c-fos protein immunohistochemical staining was carried out for each group. Results The c-los protein in group PG10,PG50,PG100 was less than that in group G10,G50,G100 （P〈0.05） respectively, Cell survival ratio was significantly decreased in group GIO（P〈0.05）,G50（P〈0.01）,G100（P〈0.01）, this change in propofol groups was more evident than glutamic acid group（P〈 0.05）. Conclusions Propofol may antagonize the neurotoxicity of excitatory amino acid.

关 键 词：谷氨酸 异丙酚 海马 神经细胞 氧化毒性 

分 类 号：R614[医药卫生—麻醉学]