毛细管电泳高频电导法测定几种中药中的丁香酚  

Determination of Eugenol in Several Chinese Herbal Medicines by Capillary Electrophoresis with High Frequency Conductivity Method

作　　者：林伟丰[1] 吴小林[2] 陈缵光[3] 李全文[3] 蔡沛祥[1] 莫金垣[1] 

机构地区：[1]中山大学化学院,广州510275 [2]中山大学附属笫三医院,广州510630 [3]中山大学药学院,广州510089

出　　处：《高等学校化学学报》2006年第11期2070-2072,共3页Chemical Journal of Chinese Universities

基　　金：国家自然科学基金(批准号:20375049);广东省自然科学基金重点项目(批准号:021808)资助

摘　　要：A method based on capillary electrophoresis with a high frequency conductivity method was(developed) for the determination of eugenol in several Chinese herbal medicines from different producing areas.In this study,some key factors for separation and detection were studied and the best analysis conditions were obtained.In the buffer system of 2.0 mmol/L triethylamine +0.74 mol/L methanol at separation potential of 18.0 kV,the optimal separation and detection was obtained.Under the chosen conditions,eugenol was eluted within 7 min.The linear range of eugenol was 2.0—540 μg/mL,the limit of detection was 0.6 μg/mL,and the recovery ranged from 93.3% to 106%.A method based on capillary electrophoresis with a high frequency conductivity method was developed for the determination of eugenol in several Chinese herbal medicines from different producing areas. In this study, some key factors for separation and detection were studied and the best analysis conditions were obtained. In the buffer system of 2.0 mmol/L triethylamine ＋ 0. 74 mol/L methanol at separation potential of 18.0 kV, the optimal separation and detection was obtained. Under the chosen conditions, eugenol was eluted within 7 min. The linear range of eugenol was 2.0-540 μg/mL, the limit of detection was 0. 6 μg/mL, and the recovery ranged from 93.3% to 106%.

关 键 词：毛细管电泳 高频电导检测 非接触式电导检测 丁香酚 中草药 

分 类 号：O657[理学—分析化学]