细胞吞噬表面电荷不同的硅纳米颗粒的研究  

Studies on the Cellular Uptake of SiNPs with Different Surface Charges

作　　者：邢新丽[1] 何晓晓[1] 王柯敏[1] 彭姣凤[1] 谭蔚泓[1] 

机构地区：[1]湖南大学化学生物传感与计量学国家重点实验室

出　　处：《高等学校化学学报》2006年第11期2076-2078,共3页Chemical Journal of Chinese Universities

基　　金：国家自然科学基金重点项目(批准号:20135010);国家重点基础研究发展规划项目(批准号:2002CB513110);科技部国际合作重点项目(批准号:2003DF000039);国家自然科学基金(批准号:20405005)资助

摘　　要：The uptake of silica nanoparticles(SiNPs) with positive surface charge and negative surface charge by HepG cells,MCF-7 cells and L-02 cells were investigated by using the RITC doped in SiNPs as a synchronous fluorescent signal indicator.The experimental results show that the uptake of SiNPs by the three kinds of cells were all a concentration-,charge-,time-,and serum-dependent endocytic process.To the same kind of SiNPs,the(uptake) by the three kinds of cells all enhanced with increasing of the SiNPs concentration and incubation time and gradually reached saturation.However,the effect of serum was different to the uptake of SiNPs with different surface charge.The results display that serum existed in culture medium decreased the cellular uptake of NSiNPs.While the serum existed in culture medium would not affect the cellular uptake of PSiNPs.These results provide a theoretical foundation for the further modification of SiNPs,which could improve the stability of SiNPs in the medium and made it be well applied to biomedicine.The uptake of silica nanoparticles（SiNPs） with positive surface charge and negative surface charge by HepG cells, MCF-7 cells and L-02 cells were investigated by using the RITC doped in SiNPs as a synchronous fluorescent signal indicator. The experimental results show that the uptake of SiNPs by the three kinds of cells were all a concentration-, charge-, time-, and serum-dependent endocytic process. To the same kind of SiNPs, the uptake by the three kinds of cells all enhanced with increasing of the SiNPs concentration and incubation time and gradually reached saturation. However, the effect of serum was different to the uptake of SiNPs with different surface charge. The results display that serum existed in culture medium decreased the cellular uptake of NSiNPs. While the serum existed in culture medium would not affect the cellular uptake of PSiNPs. These results provide a theoretical foundation for the further modification of SiNPs, which could improve the stability of SiNPs in the medium and made it be well applied to biomedicine.

关 键 词：SiO2纳米颗粒 表面电荷 细胞吞噬 

分 类 号：O657[理学—分析化学]