Association between Nef-specific CD8 T-cell responses and disease progression in HIV-1 subtype B infection  

作　　者：JIAO Yang LI Tai-sheng XIE Jing HAN Yang QIU Zhi-feng ZUO Ling-yan Thomas Mourez WANG Ai-xia 

机构地区：[1]Department of Infectious Disease, Peking Union Medical College Hospital and AIDS Research Center, Chinese Academy of Medical Sciences, Beijing 100730, China [2]Laboratory of Human and Molecular Virology, University Hospital, av. Georges Clemenceau, 14033 Caen, France

出　　处：《Chinese Medical Journal》2006年第19期1609-1615,共7页中华医学杂志（英文版）

基　　金：This study was funded by the National Nature Science Foundation of China (No. 30571641) and INCO: Phase 1/2 Clinical Testing of Clade C-based HIV-1 Candidate Vaccines in China (No. ICA4-2001-10148).

摘　　要：Background The correlation between HIV-1 Nef-specific CD8 T-cell responses and markers of HIV-1 disease progression still remains unclear. This study analysed and compared the role of HIV-1 Nef-specific CD8 T-cell responses in patients with different disease status. Methods Two groups of patients with HIV-1 subtype B infection were selected according to CD4 count and clinical manifestations： long-term nonprogresssors （LTNPs, n = 20） and advanced progressors （APs, CD4 count 〈500 cells/pl, n = 34）. Nef-specific CD8 T-cell responses were studied by interferon- T ELISpot assay against 3 pools of HIV-Nef peptides. Results Nef-specific CD8 T-cell responses did not correlate with viral load or CD4 count in all patients and no significant differences were found in the magnitude of Nef-specific CD8 T-cell responses between groups LTNPs and APs （670 SFC/106 peripheral blood mononuclear cells vs 1107 SFC/106 peripheral blood mononuclear cells, P = 0.255）. Further comparisons showed that there were also no significant correlations observed in group LTNPs, but Nef-specific CD8 T cells correlated negatively with viral load （r = -0.397, P = 0.020） and positively with CD4 count （r = 0.364, P = 0.034） in group APs. Conclusion These data suggest that different correlation patterns between Nef-specific CD8 T-cell responses and disease progression exist in LTNPs and APs. Although a negative association was observed with concurrent plasma HIV RNA in APs, Nef-specific CD8 T-cell responses might fail to play a protective role in different stages of HIV- 1 infection.Background The correlation between HIV-1 Nef-specific CD8 T-cell responses and markers of HIV-1 disease progression still remains unclear. This study analysed and compared the role of HIV-1 Nef-specific CD8 T-cell responses in patients with different disease status. Methods Two groups of patients with HIV-1 subtype B infection were selected according to CD4 count and clinical manifestations： long-term nonprogresssors （LTNPs, n = 20） and advanced progressors （APs, CD4 count 〈500 cells/pl, n = 34）. Nef-specific CD8 T-cell responses were studied by interferon- T ELISpot assay against 3 pools of HIV-Nef peptides. Results Nef-specific CD8 T-cell responses did not correlate with viral load or CD4 count in all patients and no significant differences were found in the magnitude of Nef-specific CD8 T-cell responses between groups LTNPs and APs （670 SFC/106 peripheral blood mononuclear cells vs 1107 SFC/106 peripheral blood mononuclear cells, P = 0.255）. Further comparisons showed that there were also no significant correlations observed in group LTNPs, but Nef-specific CD8 T cells correlated negatively with viral load （r = -0.397, P = 0.020） and positively with CD4 count （r = 0.364, P = 0.034） in group APs. Conclusion These data suggest that different correlation patterns between Nef-specific CD8 T-cell responses and disease progression exist in LTNPs and APs. Although a negative association was observed with concurrent plasma HIV RNA in APs, Nef-specific CD8 T-cell responses might fail to play a protective role in different stages of HIV- 1 infection.

关 键 词：NEF HIV- 1 subtype B CD8 T-cell response IFN-GAMMA ELISPOT 

分 类 号：R512.91[医药卫生—内科学]