高+Gx对猴肝细胞c-fos表达的影响  

Effect of hypergravity on expression of c-fos gene in hepatocytes of Rhesus macaque

作　　者：牛忠英[1] 张建中[1] 施生根[1] 吴斌[2] 李冬[1] 党平[1] 阚广捍[2] 

机构地区：[1]中国人民解放军第306医院,北京市100101 [2]中国航天员科研训练中心,北京市100094

出　　处：《世界华人消化杂志》2006年第28期2793-2795,共3页World Chinese Journal of Digestology

基　　金：总装备部航天医学科研基金资助项目;No.HTYK02006~~

摘　　要：目的:观察航天应急返回过程中高正加速度(+Gx)对肝脏细胞c-fos基因表达的影响．方法:选用♂猕猴(共9只)为对象,随机分为4组,对照组承受+1Gx,300 s的超重作用;实验组根据承受过载峰值的大小分为3个亚组,其承受过载峰值分别为+15Gx,200 s;+18Gx, 165 s;+21Gx,140 s．观察高+Gx对猴肝脏细胞c-fos基因表达的影响．结果:实验组肝脏细胞胞质呈现不同程度的水肿及泡状变性,c-fos基因表达明显增强,呈弥漫性细胞质内棕黄色着色;肝细胞c-fos基因表达程度随超重剂量的增加有增强趋势．对照组肝脏组织病理学改变程度明显较实验组轻微,c-fos基因表达亦明显减弱．结论:+Gx可引起猴肝脏组织细胞c-fos基因表达增强,提示有早期肝脏组织损伤．AIM： To detect the expression of c-fos in the hepatocytes of Rhesus macaque under hypergravity （＋Gx） loads. METHODS： A total of 9 male Rhesus macaques were randomly divided into experiment groups （n = 7） and control group （n = 2）. The animals in control group was exposed to ＋1Gx/300s overloads, and those in the experimental groups were exposed to the overloads of ＋15Gx/200s （n = 2）, ＋21Gx/165s （n = 2） and ＋21Gx/140s （n = 3）, respectively. Liver tissue was fixed with 40 g/L buffered formaldehyde and embedded with paraffin, and histopathological changes were observed under microscope. The expression of c-fos was detected by an immunohistochemical method. RESULTS： In the experimental groups, cytoplasmic swelling and alveolar degeneration were found in the liver; the expression of c-fos was detected in the cytoplasm of hepatocytes, with a tendency of enhancement as the dosage of hypergravity increased. In the control group, no significant histopathological changes were observed and the expression of c-fos was also remarkably decreased. CONCLUSION： Overload of gravity can induce significant injuries of the liver in Rhesus macaque.

关 键 词：高正加速度 恒河猴 c—fos表达 肝损伤 

分 类 号：R85[医药卫生—航空、航天与航海医学]