高羊茅成熟种子组织培养的影响因素研究:Ⅱ.不同因素对胚性愈伤组织继代发生的影响  

Factors affecting tissue culture responses of mature seeds in tall fescue(Festuca arundinacea Schreb.):Ⅱ.The effects of different factors on subculture production of embryogenic calli

作　　者：吴关庭[1] 胡张华[1] 陈笑芸[1] 郎春秀[1] 王伏林[1] 金卫[1] 陈锦清[1] 夏英武[2] 

机构地区：[1]浙江省农业科学院病毒学与生物技术研究所,浙江杭州310021 [2]浙江大学原子核农业科学研究所,浙江杭州310029

出　　处：《浙江农业学报》2006年第5期369-372,共4页Acta Agriculturae Zhejiangensis

基　　金：浙江绿洲生态股份有限公司风险投资项目;浙江省"151人才基金"资助项目

摘　　要：以从4个草坪型高羊茅品种成熟种子直接诱导获得的初始愈伤组织为起始材料，研究3种因素对胚性愈伤组织继代发生的影响。结果表明，继代培养基中添加BAP和硫酸铜以及提高蔗糖浓度均能促进胚性愈伤组织形成，提高胚性愈伤组织频率，其适宜浓度分别为：BAP0．1～0．2mg/L，硫酸铜2．5mg/L，蔗糖60g／L。这3种因素中，又以添加BAP的效果最好，添加硫酸铜次之。4个供试品种间胚性愈伤组织发生能力存在明显差异。The effects of three different factors on subculture production of embryogenic calli were investigated with ones induced directly from mature seeds of four tuff-type tall fescue cultivars as starting materials. The results showed that supplementing BAP and cupric sulphate and increasing sucrose concentration in subculture medium stimulated formation of embryogenic calli, with appropriate concentration being 0.1 - 0.2 mg/L for BAP, 2.5 mg/L for cupric sulphate, and 60 g/L for sucrose. Among these three factors, BAP addition was the most effective, followed by supplementation of cupric sulphate. It was also demonstrated that apparent aifferences in productivity of embryogenic callus existed among the four tested cultivats.

关 键 词：高羊茅 组织培养 胚性愈伤组织 继代 

分 类 号：S543.5[农业科学—作物学]