人类端粒酶逆转录酶hTERT Ki-67及p27^(kip1)在嗜铬细胞瘤组织的表达及意义研究  

Expression of human telomerase reverse transcriptase hTERT,Ki-67 and p27^(kip1) in pheochromocytoma and their significance.

作　　者：李健玲[1] 罗佐杰[1] 秦映芬[1] 韦敏怡[1] 梁杏欢[1] 冼晶[1] 卢德成[1] 沈昱[1] 何志恒 Jack Y.YANG Mary Qu YANG 

机构地区：[1]广西医科大学第一附属医院内分泌科,广西南宁530021 [2]the Research Division,Joslin Diabetes Center,Harvard Medical School [3]Harvard University,Massachusetts General Hospital,Harvard Medical School [4]U.S. Department of Health and Human Services,National Human Genome Research Institute

出　　处：《中国实用内科杂志：临床前沿版》2006年第11期1788-1790,共3页

基　　金：广西科技厅自然科学基金(桂科自0542085);广西教育厅科研项目(桂教科研[2004]20号)

摘　　要：目的研究人端粒酶逆转录酶(hTERT)、Ki-67及p27kip1的表达在嗜铬细胞瘤发生与发展中的作用和作为预测生物学行为标志物的价值。方法采用免疫组织化学方法检测hTERT、Ki-67及p27kip1在2000—2004年广西医科大学第一附属医院病理科的45例嗜铬细胞瘤和神经节细胞瘤及9例正常肾上腺组织中的表达。结果hTERT蛋白的表达在良性(3/31例)和可疑恶性(6/7例)以及良性与恶性肿瘤(5/7例)间的差异均有统计学意义(P<0.01)。31例良性肿瘤中26例未检测到Ki-67,而恶性肿瘤和可疑恶性肿瘤均为阳性;Ki-67与hTERT的表达呈正相关性(r=0.544,P<0.01)。恶性和可疑恶性肿瘤中未检测到p27kip1,5例良性肿瘤为阳性,所有正常肾上腺髓质标本均可检测到p27kip1的表达。p27kip1与hTERT的表达无相关性。结论端粒酶的激活在恶性嗜铬细胞瘤和神经节细胞瘤的发生发展中起着重要作用,在细胞周期调控中端粒酶可能存在不同的激活途径。hTERT和Ki-67的检测可作为鉴别良恶性嗜铬细胞瘤的手段。Objective To investigate the expression of human telomerase reverse transcriptase （hTERT）, Ki -67 and p27^kipl in pheochromocytoma and paraganglioma, and to evaluate whether the expression of hTERT, Ki -67 and p27^kipl could serve as diagnostic markers for predicting the biological behaviour of these tumours. Methods Expression of hTERT, Ki -67 and p27^kipl was determined by immunohistochemistry in 45 pheochromocytomas/paragangliomas （31 benign, 7 suspected malignant and 7 malignant） and 9 normal adrenal medulla samples. Results The hTERT was expressed in 5/7 malignant tumors, 5/7 suspected malignant tumours and 3/31 benign tumours. Malignant and suspected malignant tumors expressed more hTERT （P 〈 0. 01, respectively） than benign tumors. All benign tumors showed no immunopositivity or 〈 1% of cells stained for Ki - 67 antigen. The Ki - 67 antigen was expressed in 7 malignant tumors, 6/7 suspected malignant tumors. Ki - 67 expression was correlated with hTERT expression （ r = 0. 544, P 〈 0. 001 ）. There was no statistical difference in p27^kipl expression between benign, malignant and suspected malignant tumors. Conclusion Telomerase might play a critical role in the occurrence and development of malignant phaeochromocytomas/ paragangliomas, and there are various telomerase activated pathways in cell cycle regulation, hTERT and Ki -67 detection are useful tools to differentiate malignant from benign phaeochromocytomas/paragangliomas.

关 键 词：嗜铬细胞瘤 神经节细胞瘤 HTERT KI-67 P27^KIPL 

分 类 号：R5[医药卫生—内科学]