宜兴百合的组织培养和快速繁殖  

Tissue culture and rapid propagation of Lilium lancifolium Thunb.

作　　者：谢杰[1] 余沛涛[1] 王全喜[1] 

机构地区：[1]上海师范大学生命与环境科学学院,上海200234

出　　处：《上海师范大学学报（自然科学版）》2006年第5期76-82,共7页Journal of Shanghai Normal University(Natural Sciences)

摘　　要：以组织培养获得的宜兴百合小鳞茎为试验材料,研究了不同基本培养基、NAA与6-BA的配比、矮壮素(CCC)、2,4-D、活性炭、蔗糖及悬浮培养方法对小鳞茎增殖和膨大的影响,结果表明,最佳诱导培养基为MS培养基,有利于提高分化率的最佳配方为:MS+0．15 mg/LNAA+2．0mg/L 6-BA,小鳞茎膨大增重的最佳培养基为:MS+0．15mg/L NAA+2．0 mg/L6-BA+0．1mg/L CCC+6-8%蔗糖,培养方式为液体振荡培养优于固体培养。The reproduction and extension of bulhlets of lily was tested by the way of tissue culture. The effects of the different medium, the combination of NAA and 6-BAm, the chlormequat（CCC）, the 2,4 -D, the active carbon, the Sucrose and the methods of Suspension culture were studied in the tests. The results showed that MS medium was the most appropriate basic medium for inducement of bulblets. The best medium for the scale of lily to reproduction and extension was MS ＋ 0.15mg/L NAA ＋ 0. 20mg/L 6-BA, the most appropriate medium for adding weight of bulblets of lily was MS ＋ 0.15mg/L NAA ＋ 2.0mg/L6-BA ＋ 1. 0mg/L CCC ＋ 6 -8% Sucrose. The method of suspension culture was better than that of solide culture.

关 键 词：宜兴百合 组织培养 快速繁殖 

分 类 号：Q943.1[生物学—植物学]