HIV-1高暴露持续血清阴性者对Nef、Gag肽段库的特异性CTL应答的研究  

Analysis of Nef,Gag-specific CTL responses in HIV-1 highly exposed persistently serongeative individuals

作　　者：焦洋[1] 谢静[1] 韩扬[1] 邱志峰[1] 左玲燕[1] 王爱霞[1] 李太生[1] 

机构地区：[1]中国医学科学院 中国协和医科大学 北京协和医院感染内科,北京100730

出　　处：《中华微生物学和免疫学杂志》2006年第10期890-893,共4页Chinese Journal of Microbiology and Immunology

基　　金：首都医学发展科研基金艾滋病项目(2003-1006);欧盟艾滋病疫苗项目(ICA4-CT-2002-10042)

摘　　要：目的研究中国HIV-1高暴露持续血清阴性(highly exposed persistently seronegative,HEPS)者的Nef、Gag特异性细胞毒性T淋巴细胞(cytotoxic T lymphocyte,CTL)应答特点,探讨HIV-1特异性CIL应答在这类特殊人群中抵抗感染的作用机制。方法选取10例HIV-1高暴露持续血清阴性者,11例经性接触感染且从未接受抗病毒治疗的HIV/AIDS患者及4例未经暴露的健康志愿者。以覆盖HIV-1gag全长和部分nef的14个肽段库为刺激原,应用IFN-γELISPOT法测定3组人群的特异性CTL应答,并对3组的应答强度、宽度以及对肽段库识别比例进行比较。结果50％(5/10)的HEPS,100％(11/11)的HIV/AIDS患者均存在Nef及Gag特异性CTL应答,而4例健康对照均为阴性。存在应答的HEPS者对14个肽段库的平均应答强度和宽度分别是HIV/AIDS患者的4．3％和37．7％。在HEPS者中主要识别的肽段库均为HIV/AIDS患者中识别比例相对较低的肽段库。结论与HIV/ AIDS患者相比,HEPS者中的HIV-1特异性CTL应答存在着不同的特点和规律,可能在保护机体免于HIV-1感染中发挥着重要作用。Objective To study the characteristics of HIV-1 Nef, Gag-specific cytotoxic T lymphocyte （CIL） responses in Chinese HIV-1 highly exposed persistently seronegative（HEPS）individuals and to evaluate the roles of the specific responses in persistent nontransmission of HIV- 1. Methods HIV- 1 specific CTL responses to 14 overlapping peptide pools encompassing products of the HIV-1 gag and nef genome were tested by INF-γ enzyme-linked immunospot （ELISPOT） assay in 10 HEPS individuals from discordant couples, 12 HIV/AIDS patients （by sexual transmission）and 4 healthy subjects without any exposure. The recognition rotes, average magnitude and breadth of the specific responses were compared among the three cohorts. Results It was confimed that Gag and Nef-specific T lymphocytes were present in HEPS individuals, which were absent in healthy control. However, the responses were much less frequent （50% vs 100% ）, of a lower magnitude（4.3% ）, and of narrower breadth （37.7%） than in HIV/AIDS patients. Main poptides recognized by HEPS individuals were less frequently recognized by HIV/AIDS patients. Conclusion Differences exist in the characteristics of Nef, Gag-specific responses between HEPS individuals and HIV/AIDS patients. CIL responses may play a key role in the protection from HIV-1 infection in HEPS individuals.

关 键 词：人免疫缺陷病毒1型 细胞毒性T淋巴细胞 高暴露持续血清阴性 

分 类 号：R512.91[医药卫生—内科学]