中国HIV-1感染人群中和抗体水平与疾病进展关系研究  

Study on neutralizing antibody levels and disease progression in HIV-1 infected subjects in China

作　　者：王倩[1] 尚红[1] 张子宁[1] 韩晓旭[1] 姜拥军[1] 王亚男[1] 刁莹莹[1] 代娣[1] 

机构地区：[1]中国医科大学附属第一医院卫生部艾滋病免疫学重点实验室,沈阳110001

出　　处：《中华微生物学和免疫学杂志》2006年第10期894-897,共4页Chinese Journal of Microbiology and Immunology

基　　金："十五"国家科技攻关课题资助项目(2004BA719A12);国家自然科学基金(30471548)

摘　　要：目的研究中国HIV感染者、AIDS患者及疾病长期不进展者(LTNP)HIV-1中和抗体水平,探讨中和抗体对HIV-1感染疾病进程的影响。方法提取正常人外周血单个核细胞(PBMC),应用HIV-1 SF33毒株进行Single-Round PBMC中和试验,流式细胞仪分析CD4^+ T细胞内p24-Ag表达。A least-squares regression计算中和抗体滴度。结果HIV感染者、AIDS患者及LTNP中和抗体平均滴度分别为1：29．09、1：22．07、1：154．37。LTNP中和抗体滴度显著高于HIV感染者(t=-2．220,P＜0．05)及AIDS患者(t=-3．812,P＜0．01)；HIV感染者与AIDS患者中和抗体滴度差异无统计学意义(t= 0．424,P＞0．05)。结论中和抗体水平影响中国HIV感染者疾病进展,高水平中和抗体有利于延缓病情进展。Objective To study neutralizing antibody levels of HIV-infected individuals, AIDS patients and long-term nonprogressors （LTNP） in China, explore the influence of HIV-1 neutralizing antibody on disease progression of HIV-infected individuals. Methods Peripheral blood mononudear cells were isolated from HIV-seronegative donors. Single-round PBMC neutralization assays were performed with HIV-1 isolate SF33. The intracellular p24-Ag expression of CD4 ^＋ lymphoeytes was analyzed with flow cytometry. The titer of neutralizing antibody was ealeulated by a least-squares regression analysis. Results The geometric mean titers for HIV-infeeted individuals, AIDS patients and LTNP were respectively 1：29.09, 1：22.07 and 1 ： 154.37. The titers of neutralizing antibody for LTNP were signifieantly higher than that for HIV-infeeted individuals （ t = -2.220, P 〈0.05） and AIDS patients （ t = -3.812, P 〈 0.01 ）. But the neutralizing antibody titers showed no significant ditterenee（ P 〉 0.05） between HIV-infected individuals and AIDS patients. Conclusion The levels of neutralizing antibody had influence on disease progression of HIV-infected individuals. The high levels of neutralizing antibody may arrest disease from progressing.

关 键 词：HIV-1 中和抗体 LTNP 

分 类 号：R512.91[医药卫生—内科学]