附睾精子抽吸术结合卵细胞浆内单精子注射治疗阻塞性无精子症所致男性不育的研究  

Epididymal sperm aspiration combined with intracytoplasmic sperm injection in the treatment of obstructive azoospermia induced male infertility

作　　者：钟兴明[1] 韦相才[1] 姚小涛[1] 李庆琨[1] 陈瑞玲[1] 陆艳华[1] 

机构地区：[1]广东省计划生育科学技术研究所广东省计划生育专科医院,510600

出　　处：《中国妇幼保健》2006年第21期3006-3008,共3页Maternal and Child Health Care of China

摘　　要：目的：探讨附睾精子抽吸术（ESA）结合卵细胞内单精子注射（ICSI）技术治疗阻塞性无精子症所致男性不育的治疗效果。方法：选择2002年1月-2003年12月到我院治疗不育症确诊为阻塞性无精子症男性不育患者，采用ESA方法吸取男性附睾液，分离精子用于ICSI；同时按常规体外受精-胚胎移植方法（IVF—ET），采用GnRH—α＋FSH／hMG＋hCG促排卵方案对女性进行促排成熟卵细胞（MII）用于显微注射授精，受精卵体外培养3d后移植回子宫内。结果：采用MESA结合ICSI技术治疗32周期阻塞性无精子症所致不育的夫妇，所获成熟卵（MII）162个，受精率66．28％，卵裂率62．21％，临床妊娠率31．20％。结论：采用ESA结合ICSI技术治疗阻塞性无精子症所致男性不育获得良好的效果，该方法为阻塞性无精子症男性不育患者提供了一种快速、方便、无痛、有效的治疗方法。Objective： To investigate the efficacy of intracytoplasmic sperm injection （ICSI） combined with epididymal sperm aspiration （ESA） in the treatment of obstructive azoospermia induced male infertility. Methods： From January 2002 to December 2003, 12 cycles were selected, ESA were adopted to aspirate epididymal sperms for ICSI, and ovarian stimulation was achieved on routine in vitro fertili- zation - embryo transfer （ IVF - ET） protocols. All metaphase Ⅱ （ M Ⅱ） oocytes were selected for micro - sperm injection, the embryos were transferred back to the uterine 3 days after insemination. Results： 62 M Ⅱ oocytes were injected in 12 cycles, the insemination rate and embryo cleavage rate was 66. 28% and 62. 21% respectively, two couple pregnancy and clinic pregnant rate was 20%. Conclusion： ESA combined with ICSI is an effective approach for the treatment of obstructive azoospermia induced male infertility and also a rapid, convenient method for clinic male infertility patients.

关 键 词：附睾精子抽吸术 卵细胞浆单精子注射 阻塞性无精子症 男性不育 

分 类 号：R711.6[医药卫生—妇产科学]