超声提取绞股蓝总皂苷及抗氧化作用的研究  

The Study on Ultrasound Extraction and Antioxidative Effect of Gypenosides

作　　者：张慧丽[1] 宋有涛[1] 辛如[1] 李玉[1] 刘宏生[1] 

机构地区：[1]辽宁大学生命科学系,辽宁沈阳110036

出　　处：《辽宁大学学报（自然科学版）》2006年第4期346-348,共3页Journal of Liaoning University：Natural Sciences Edition

摘　　要：通过正交实验优化提取确定绞股蓝总皂苷的最佳条件及抗氧化作用.采用L9(3)4确定温度、超声波功率、提取时间、80%的甲醇溶液用量;用最优方案大量提取绞股蓝总皂苷给老龄大鼠灌胃,然后再通过测定给药后老龄大鼠红细胞超氧化物歧化酶抗氧化酶含量.通过实验得到绞股蓝总皂苷的的最佳条件:60℃,20 kHz超声波功率,80%的甲醇溶液52 mL回流提取3次,每次130 m in;绞股蓝总皂苷40、50 mg/kg两剂量组均能明显升高老龄大鼠红细胞SOD活力.The design orthogonal experiment inspects differently withdraws the plan to determine the result the influence. The result indicated, 50℃, 20 kHz ultrasonic wave power, the 80% methyl alcohol solution 52 mL backflow withdraws 3 times, each time 130 min best withdraws the plan twists the stock blue total glucoside in the observation to the old age rat＇s antioxidation partial experiments in, after determination to medicine rat red blood cell hyperoxide mutase （SOD） content, proved twists the stock blue total glucoside to have reduces the free radical vigor, the enhancement old age big mouse organism antioxidation ability function.

关 键 词：正交实验 超声波 绞股蓝总皂苷 抗氧化作用 

分 类 号：R282.710.5[医药卫生—中药学] R285.5[医药卫生—中医学]