水稻胞质FBA基因在鱼腥藻7120中的表达及其对光合作用的调控  

Expression rice cytosol fructose 1,6-bisphosphate aldolase in Anabaena sp. PCC 7120 and explore its regulation function in photosynthesis

作　　者：冯燕[1] 陈晓[1] 施定基[1] 王全喜[1] 

机构地区：[1]上海师范大学生命与环境科学学院,上海200234

出　　处：《植物研究》2006年第6期691-698,共8页Bulletin of Botanical Research

基　　金：上海市教委重点科研项目(05ZZ15)

摘　　要：光合作用包括了一系列复杂的反应,其中碳固定反应是光合作用调控的核心环节。果糖-1,6-二磷酸醛缩酶(FBA)是Calvin循环中固定CO2后第一个催化三碳化合物转变为六碳化合物的酶,在光合作用中有着重要的作用。近年来,反义技术进一步地证明了FBA在加速碳固定反应方面有着非常大的潜力。本论文通过过表达技术来研究提高FBA活性是否能加速碳固定反应。将水稻胞质FBA嵌合基因转入鱼腥藻7120,通过相应的抗生素筛选及PCR鉴定后,确定得到了稳定遗传的转基因藻。对转基因藻和野生藻进行FBA活性及生理活性的测定后发现:转基因藻中FBA的活性比野生藻提高了31.2%;细胞生长速率比野生藻提高了24.4%;净光合与真实光合分别比野生藻提高了19.2%和20.6%。以上结果证明,在鱼腥藻体内特异的提高非调控酶FBA的水平,能在一定程度上提高转基因藻的光合活性,并且能够提高转基因藻的细胞增长效率。为进一步研究FBA在光合碳流量中的调控机理提供实验依据。Photosynthesis contains a complex series of reactions, and carbon fixation is the core reaction of photosynthesis regulation. Fructose 1,6-bisphosphate aldolase （FBA） serves as the first enzyme to convert 3C into 6C compound after CO2 been fixed. So FBA is a very important enzyme in photosynthetic process. Lately, at the use of antisense, the results of which also unveils that FBA has a strong potential in accelerate the rate of carbon fixation. In this article, we try to use gene overexpress technology to explore the function of FBA. The FBA gene from rice cytoplasm was transferred into filamentous, heterocystour cyanobacterium Anabaena sp. PCC 7120. Through antibiotic selection, we finally got the strain that could constantly express this meric gene. Cells were grown in BG-11 medium. The growth parame- ters, photosynthesis activity and enzyme activity of FBA were measured and compared between transgen- ic and wild type cells. The result showes that, compare to the wild strain, the transgenic strain showed a 31.2% higher in FBA activity, 24. 4% higher in growth pace, 19.2% higher in net photosynthesis, and 20. 6% much higher in ture photosynthesis. All the results prove that the increase of FBA level in Anabaena sp. PCC 7120 could help increase the cell grow rate. help enhance the photosynthesis activity in transgenic strain and could Our research could be an experiment support for the possible regulation role of FBA.

关 键 词：果糖-1 6-二磷酸醛缩酶(FBA) 转基因 光合调控 

分 类 号：Q949.22[生物学—植物学]