NaHCO_3对氧惊厥潜伏期的影响  

The effect of 5 % NaHCO_3 on the latency of hyperbaric oxygen-induced convulsion

作　　者：彭忠将[1] 韩晓琛[1] 孙学军[1] 蒋春雷[1] 刘刊[1] 李润平[1] 黄俊龙[1] 

机构地区：[1]第二军医大学海军医学系,上海200433

出　　处：《中华航海医学与高气压医学杂志》2006年第5期270-272,共3页Chinese Journal of Nautical Medicine and Hyperbaric Medicine

基　　金：第二军医大学联合研究基金(200301)

摘　　要：目的观察NaHCO3对氧惊厥潜伏期的影响,以探讨内源性CO2与氧惊厥的关系。方法在复制小鼠氧惊厥模型的基础上,行为学方法测定不同压力下氧惊厥潜伏期。小鼠24只,随机分为6组:3、4、5 ATA下,分别设生理盐水组、5％NaHCO3组,每组4只。另取小鼠15只,随机分为3组:常压对照组、生理盐水组和5％NaHCO3组,每组5只。后2组在4 ATA医用纯氧下暴露18 min后,测定脑组织氧化产物丙二醛(maleic dialdehyde,MDA)含量。结果(1)与生理盐水组相比,5 ATA下,5％NaHCO3组的氧惊厥潜伏期明显延长(P<0．01);4 ATA下,5％NaHCO3组的氧惊厥潜伏期也延长(P< 0．01);3 ATA下,由于120 min后小鼠仍未发生惊厥,所以不能作出判定。(2)4 ATA高压氧暴露18 min后,5％NaHCO3组与生理盐水组的脑组织MDA含量明显低于常压对照组;5％NaHCO3组的脑组织MDA含量明显低于生理盐水组(P<0．05)。结论5％NaHCO3可以延长氧惊厥潜伏期,减轻脑组织氧化损伤程序。Objective To observe the effect of 5% NaHCO3 on the latency of hyperbaric oxygeninduced convulsion in mice, and to explore the relationship between endogenesis carbon dioxide and hyperbaric oxygen-induced convulsion. Methods First, twenty-four mice were randomly divided into six groups： 5% NaHCO3 and normal saline （NS） groups under 3 ATA, 4 ATA, 5 ATA, respectively. The animals were given intraperitoneally 5% NaHCO3 or NS. The effect of 5% NaHCO3 on the latency of hyperbaric oxygen-induced convulsion under different pressures was observed. Second, fifteen mice were randomly divided into three groups： normal pressure group, NS group, 5% NaHCO3 group. Changes in the content of maleic dialdehyde （MDA） were measured after 18 rain under 4 ATA pressure of pure oxygen. Results （1） There was a significant difference in the latency of hyperbaric oxygen-induced convulsion between 5% NaHCO3 group and NS group under 5 ATA and 4 ATA （P 〈 0.01）. But difference between 5% NaHCO3 group and NS group under 3 ATA pressure was not found, for the animals didn＇t present convulsion after 120 min. （2） Under 4 ATA pressure, after 18 min, there was a significant difference of the MDA content in the cortex, hippocampus, and striatum between the NS group and the normal pressure group, as well as between the 5% NaHCO3 group and the normal pressure group （P 〈 0.05）. The MDA content in the cortex, hippocampus, and striatum in the 5% NaHCO3 group was significantly lower than that of the NS group （P 〈 0.05）. Conclusions It was suggested that 5% NaHCO3 could lengthen the latency of hyperbaric oxygen-induced convulsion, and abate the oxidation damage of the brain tissues.

关 键 词：高压氧 惊厥 碳酸氢钠 二氧化碳 

分 类 号：R459.6[医药卫生—治疗学]