绿色荧光蛋白GFP基因与新生隐球菌荚膜基因的融合表达系统  

Construction of the fusion expression system of Cryptococcus neoformans capsular gene and green fluorescent protein gene

作　　者：潘炜华[1] 廖万清[1] 刘晓刚[1] 朱元杰[1] 霍克克[2] 

机构地区：[1]第二军医大学附属长征医院皮肤科,上海200003 [2]复旦大学遗传学研究所,上海200433

出　　处：《中国真菌学杂志》2006年第4期193-196,共4页Chinese Journal of Mycology

基　　金：国家自然科学基金资助项目(39570043)

摘　　要：目的构建新生隐球菌荚膜基因与绿色荧光蛋白的融合表达系统。方法PCR法扩增CAP60基因片段,测序验证其准确性。将其与多个必需基因共同连入穿梭质粒。结果获得6150bps大小的质粒,该质粒含有荚膜基因启动子、终止子及荧光蛋白的基因。结论将新生隐球菌荚膜基因与荧光蛋白基因融合表达,将会有利于对荚膜的生化合成途径作进一步研究。Objective To construct a fusion expression system of capsular gene of Cryptococcus neoformans and green fluorescent protein （GFP） gene. Methods The capsular gene CAP60 was obtained by PCR method and sequenced for verification, then the capsular gene and other essential genes was linked into shuttle vector by ligase. Results We got a 6150bps plasmid, including CAP60 gene, promotor, terminator and fluorescent protein gene. Conclusion The fusion expression of capsular gene of Cryptococcus neoformans and GFP gene will help to research the biochemical synthesis pathway of the capsular polysaccharide.

关 键 词：新生隐球菌 融合表达 绿色荧光蛋白 

分 类 号：R379.5[医药卫生—病原生物学]