臭氧应激对人支气管上皮细胞上皮钙粘素表达的影响  

Effects of ozone-stress on E-cadherin expression in human bronchial epithelial cells

作　　者：白洪波[1] 管茶香[1] 刘永平[1] 张敏[1] 郑世平[1] 崔艳茹[1] 刘惠君[1] 

机构地区：[1]中南大学湘雅医学院生理学系

出　　处：《中国临床药理学与治疗学》2006年第9期999-1002,共4页Chinese Journal of Clinical Pharmacology and Therapeutics

基　　金：国家自然科学基金项目(№39800053)

摘　　要：目的:观察人支气管上皮细胞(humanbronchial epithelial cells,HBECs)上皮钙粘素(E-cad-herin,E-cd)的表达,探讨E-cd在维持气道损伤修复过程中的变化及意义。方法:分别采用免疫细胞化学染色和RT-PCR的方法检测HBECs E-cd及HBECsE-cd mRNA的表达。结果:臭氧(ozone,O3)应激可使HBECs膜上E-cd的表达减少,胞浆内E-cd表达增加(P<0.05)。随着O3应激停止后的时间延长,膜上E-cd表达逐渐增加,胞浆内E-cd表达则减少。在停止O3应激后的第32 h E-cd的表达恢复正常。O3应激对HBECs E-cd mRNA的表达无明显影响(P>0.05)。结论:HBECs有E-cd表达,O3应激影响HBECs E-cd的分布。AIM： To explore the expression of Eeadherin （E-ed） in the human bronchial epithelial cells （HBECs） and their role in the damage and injury of the airway. METHODS： The E-cd protein expression and the E-cd mRNA of HBECs were determined by immunocytochemistry analysisand RT-PTR analysis, respectively. RESULTS： Ozone-stress caused a decrease in the HBECs membrane E-cd expression and an increase the cytoplasmic E-cd expression （ P 〈 0.05）. These changes returned to normal 32 h after the ozone-stress was withdrawn. O3-stress had little effect on the expression of HBECs E-cd mRNA （ P 〉 0.05）. CONCLUSION： E- cd is distributed on HBECs. O3-stress decreases the membrane E-cd expression but increases the eytoplamie E-cd expression. The change of E-cd expression may mediate HBECs injuries induced by O3-stress.

关 键 词：上皮钙粘素 支气管上皮 臭氧 

分 类 号：R965.2[医药卫生—药理学] R562.2[医药卫生—药学]