胰岛素治疗对糖尿病大鼠骨骼肌内脂肪含量和CPTI-β基因表达的影响  

Effects of insulin treatment on content of intramyocellular triglyceride and expression of CPTI-βgene in skeletal muscle of diabetic mellitus rats

作　　者：吴迁[1] 陈璐璐[1] 王保平[1] 魏翠凤[1] 周慜[1] 李燕[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院内分泌科

出　　处：《中国临床药理学与治疗学》2006年第9期1065-1068,共4页Chinese Journal of Clinical Pharmacology and Therapeutics

摘　　要：目的:观察胰岛素治疗对糖尿病(DM)大鼠骨骼肌内甘油三酯(TGm)含量和肉碱-软酯酰转移酶I-β(CPTIβ-)基因表达的影响。方法:DM大鼠随机分为DM高脂饮食组(DH,n=10)和DM高脂饮食胰岛素治疗组(DHI,n=10),正常Wistar大鼠作为正常饮食对照组(NC,n=10)。10周后检测空腹血浆葡萄糖(FPG)、胰岛素(FPI)、游离脂肪酸(FFAs)、甘油三酯(TG)和TGm,并根据FPG和FPI计算HOMA2-IR。RT-PCR法检测骨骼肌CPTIβ-基因表达水平。结果:DH组的HOMA2-IR、TG、FFAs及TGm均较NC组明显增加(P<0.01),CPTIβ-表达减少(P<0.01)。与DH组相比,DHI组胰岛素抵抗减轻(P<0.05),FFAs明显降低(P<0.01),但TG未降低(P>0.05),且TGm含量进一步增加(P<0.01),但CP-TIβ-表达水平明显增加(P<0.01)。结论:DM大鼠TGm含量增加并伴有CPTIβ-表达降低,胰岛素虽然能增加TGm,但可以增加CPTIβ-表达,从而可能改善骨骼肌中的脂肪代谢。AIM： To study the effect of insulin administration on the content of triglyceride and the expression of carnitine palmitoyl transferase I-β （CPTI-β） gene in skeletal muscle of diabetic mellitus （DM） rats. METHEDS： Wistar rats were randomly divided into normal control group （NC, n = 10） , diabetes with high fat diet （DH, n = 10） , and diabetes with high fat diet plus insulin therapy （DHI, n = 10）. The fasting plasma glucose （FPG）, insulin （FPI）, free fatty acids （FFAs）, triglyceride （TG） and the TGm （intramyocellular triglyceride） and the expression of CPTI-β gene in skeletal muscle were measured. HOMA2-1R was computed on the basis of FPI and FPG. RESULTES： Compared with NC group, HOMA2-IR, TG and FFAs levels in the plasma and TGm contents of DH group were significantly higher （P 〈 0.01）, and the expression of CPTI-β gene was decreased （ P 〈 0.01）. Compared with DH group, insulin administration didn＇ t normalize TG （ P 〉 0.05）, but FFAs were decreased （ P 〈 0.01 ）, and TGm were increased （P 〈 0.01）, although it improved insulin sensitivity of DHI group （ P 〈 0.05）, and the expression of CPTI-β were increased （ P 〈 0.01 ）. CONCLUSION： TG is accumulated in the skeletal muscle of DM rats, and the expression of CPTI-β is decreased, too. Insulin administration is able to up-regulate CPTI-β transcription, although it can increase the content of TGm as well. This phenomenon suggests insulin can ameliorate triglyceride metabolize in the DM rats.

关 键 词：糖尿病 胰岛素 甘油三酯 肉碱-软脂酰转移酶I—β 骨骼肌 

分 类 号：R965.2[医药卫生—药理学] R587.1[医药卫生—药学]