捕食应激致大鼠的持续性情绪唤醒障碍  

Persistent emotionality disorder in rats with predator stress

作　　者：王庆松[1] 吴渝宪[1] 王伟文[1] 林杭[1] 曹仁存[1] 

机构地区：[1]解放军成都军区成都总医院神经内科,四川省成都市610083

出　　处：《中国临床康复》2006年第42期57-60,共4页Chinese Journal of Clinical Rehabilitation

基　　金：成都军区十五医药卫生科研基金课题(04A011)~~

摘　　要：目的:探讨严重心理应激是否可引发实验大鼠较长时间的情绪唤醒水平障碍,以建立较理想的创伤后应激障碍动物模型。方法:实验于2004-01/2005-06在解放军成都军区成都总医院完成。选择雄性Wistar大鼠135只,成组设计随机分为捕食应激组75只和正常对照组60只。先将大鼠放入捕食应激实验箱的小笼中,再将一只饥饿的猫放入实验箱中,使猫与大鼠无生理伤害性接触,致大鼠出现轻微惊恐性颤动及因惊吓所致呼吸急促而快速鼻翼煽动6～8min;正常对照组大鼠除不接触猫外,其他处理程序相同。并通过大鼠运动活性、探究行为、拒俘反应性和高架十字迷宫实验观测应激后4个月内实验大鼠情绪唤醒水平改变,同时以Morris水迷宫法检测其空间学习和记忆能力。采用SPSS9.0软件进行单因素或双因素方差分析,以及各组均数的S-N-K法和Tamhane’sT2法检验。结果:参与实验大鼠135只在实验过程中无死亡,均进入结果分析。①与正常对照组相比,捕食应激后1个月内应激大鼠旷场爬越行为明显减少[(71.5±13.5),(96.8±18.4)分,P<0.05];4个月时后肢性站立仍明显减少[(11.4±2.1),(17.9±3.3)分,P<0.05]。②与正常对照组相比,高架十字迷宫实验中应激大鼠进入开臂次数百分比和开臂滞留时间百分比均明显降低[(4.8±0.9)%,(20.7±3.7)%,P<0.01];[(0.9±0.2)%,(6.6±1.2)%,P<0.01]。③与正常对照组相比,拒俘反应性则在应激后2个月内明显增加[(2.4±0.5),(1.5±0.4)分,P<0.05]。④与正常对照组相比,捕食应激后第1天应激大鼠在Morris水迷宫空间觅向训练中潜伏期明显延长[训练15次时为(32±6),(17±3)s,P<0.05],在空间定向能力测试时,应激大鼠水迷宫的4个象限中无目的漫游所花费时间百分比差异无显著性意义。应激后2周时大鼠空间学习和记忆能力测试无明显改变。结论:单纯捕食应激成功诱发了实验大鼠持续性运动活性减少、探究行为AIM： To investigate whether or not severe psychological stress may facilitate the persistence of abnormal emotionality in rats, so as to establish an ideal animal model of posttraumatic stress disorder （PTSD）. METHODS： The experiment was performed from January 2004 to June 2005 in Chengdu General Hospital of Chengdu Military Area Command of Chinese PLA. 135 male Wistar rats were divided randomly into 2 groups： predator stress group （PS group, n=75） arid normal control group （NC group, n=60）. Rats in the PS group were put in a predator stress case, in which there was a starved cat for non-injurious contacting until the rats were slightly frightened and twitched iwth nares flaring due to the polypnea for 6-8 minutes. Rats in the NC group were prevent from approaching to cat based on the procedures conducted in the PS group. The changes in emotionality of experimental rats were tested by locomotor activity, exploratory behavior, capture-resistance and elevated plus-maze （EPM） test at 4 months after stress. Meanwhile, the spatial learning and memory ability were measured by the Morris water maze training. Statistical analysis was performed by one-factor or two-factor analysis of variance （ANOVA） using the S-N-K test or Tamhane＇s T2 test （Sigma Stat, Version 9.0 for Windows, SPSS, Inc., Chicago, IL）. RESULTS： A total of 135 rats were involved in the analysis of results without death. ① Compared with the normal control group, rats in the PS group displayed a substantial decrease in number of creeping within one month after predator stress [（71.5±13.5）, （96.8±18.4） points,P 〈 0.05], and there was a significant diminution in standing on hind limbs after 4 months [（11.4±2.1 ）, （17.9±3.3） points,P 〈 0.05]. ② Compared with the normal control group, the percentages of open-arm entries and the percentages of open-ann retention time of rats in the PS group in EPM test were obviously decreased [（4.8±0.9）%,（20.7±3.7）% ,P 〈 0.01];[（0.9±0.2）%, （6.

关 键 词：应激障碍 创伤后 捕食应激 情绪唤醒 动物模型 

分 类 号：R338.64[医药卫生—人体生理学]