薄层扫描法测定食物中胆固醇的含量  

Determination of Cholesterol in Food by TLC

作　　者：罗健 张学新 钟爱 

出　　处：《广东药学院学报》1996年第2期88-90,共3页Academic Journal of Guangdong College of Pharmacy

摘　　要：本文探讨食物中胆固醇的薄层光密度法的测定。用硅胶Ｇ与０．３％ＣＭＣ－Ｎａ按１：２．２比例制成薄层板，石油醚－乙醚－冰醋酸（７：３：０．１）为展开剂，５％磷钼酸乙醇溶液显色，日本岛津ＣＳ－９１０型薄层扫描仪定量扫描。测得精密度变异系数（ＲＳＤ％）为２．９２，稳定性变异系数（ＲＳＤ％）为２．３５，回收率在８７．９５～９４．２９％范围内。与气相色谱法（其回收率在９２％～１０３％范围）比较，结果相接近。该法简便易行，重现性、稳定性好。Cholesterol in foods were determined by TLC technique. The thin layer sheetwas made with silica geI G and 0.3% CMC-Na (1:2.2 v/v). The food samples were developedin a mobile sYstem of petroleum-ether-glacial acetic acid (7:3:0.1 v/v), and 5% phosphoricacid alcohol solution was used for the coloration of samples. The results showed that the reap-pearance was 2.92% (RSD%) and the stability was 2.35% (RSD%). The recoverv rate was87.95～94.29%. Using GC, we found a similar result. It could be concluded that this method isa simple one and has good reappearance and stability.

关 键 词：食物 胆固醇 薄层光密度法 

分 类 号：R151.3[医药卫生—营养与食品卫生学]