流式细胞仪分离人早孕绒毛外滋养细胞及鉴定  

Isolation and characterization of human first-trimester extravillous trophoblst using flow cytometry.

作　　者：侯蕾[1] 陈必良[1] 张易[2] 柏露[1] 

机构地区：[1]解放军第四军医大学西京医院妇产科,西安710032 [2]解放军第三医院,宝鸡市721000

出　　处：《中国优生与遗传杂志》2006年第11期42-43,46,F0002,共4页Chinese Journal of Birth Health & Heredity

摘　　要：目的用流式细胞仪分离早期绒毛外滋养细胞,以便对其功能进行进一步研究。方法用胰蛋白酶消化法得到早期绒毛细胞,用流式细胞仪分离纯化绒毛外滋养细胞。所得的细胞用免疫细胞化学,光镜,透射电镜检以及W esten-b lot方法鉴定。结果使用流式细胞仪成功的分离了绒毛外滋养细胞,纯度超过98%。结论此方法可快速准确大量获得绒毛外滋养细胞。Objective： Prepareation of highly purified human extravillous trophoblast from first -trimester chorionic villous by using flow cytometry and characterization. Methods： Obtained trophoblast cells from first - trimester chorionic villi （,5 - 10） weeks sequential triypsinization and purified, isolated by flow cytometry. All trophoblast was identitied by lightmicroscope, immunocytochemistry, transmission electron microscope and westen blot. Results： Flow cytometry can obtain high purityed trophoblast subpopulations. Conclusion ： This method is can obtain high purified trophoblast subpopulation from first - trimester chorionic viii.

关 键 词：绒毛外滋养细胞 流式细胞仪 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]